Abstract：Objective: To investigate the effects of balsam pear saponins on the growth of human malignant melanoma SKMEL-2 cells and JAK/STAT5 pathway. Methods: Cultured human malignant melanoma SKMEL-2 cells were treated with balsam pear saponins at concentrations of 1, 2.5, 5, 7.5 and 10 mg/mL. The proliferation of SKMEL-2 cells was detected by CCK-8 method after 24, 48 and 72 hours, respectively, and the appropriate concentration and time of drug action were screened. SKMEL-2 cells cultured in vitro were randomly divided into three groups: control group, balsam pear saponin group and cisplatin group. After drug treatment, CCK-8 was used to detect cell proliferation, cell viability was measured, and cell apoptosis was detected by flow cytometry, the apoptotic rate was measured. The expression levels of caspase 3, Bcl-2, Bax, JAK, p-JAK, STAT5 and p-STAT5 were detected by immunoblotting (WB). Results: The inhibitory effect of the drug was higher at 48 hours and 72 hours than that at 24 hours, while the inhibitory effect was basically the same at 48 hours and 72 hours. The proliferation of SKMEL-2 was inhibited by balsam pear saponins at concentrations of 1-5, 7.5 and 10 mg/mL, and the inhibition increased with the increase of concentration, but after 48 and 72 hours, the inhibitory effect tended to be stable when the concentration reached 5 mg/mL. SKMEL-2 cells were treated with balsam pear saponins at a concentration of 5 mg/mL for 48 hours. Compared with control group, the relative survival rate, expressions of p-JAK, p-STAT5 and Bcl-2 in balsam pear saponin group and cisplatin group decreased significantly (P<0.05), apoptotic rate, caspase 3 and Bax expression were significantly increased (P<0.05), there was no significant difference in JAK and STAT5 expressions (P>0.05). There was no significant change in each index between balsam pear saponin group and cisplatin group (P>0.05). Conclusion: Momordica charantia saponins can inhibit the growth of SKMEL-2 cells, promote their apoptosis. It may be the mechanism that momordica charantia saponins can down-regulate the activity of JAK/STAT5 pathway, up-regulate the expressions of caspase 3 and Bax, and inhibit the expression of Bcl-2.
黄翠敏, 叶秀群, 骆月姬. 苦瓜皂苷对人恶性黑素瘤SKMEL-2细胞增殖凋亡及JAK/STAT5通路的影响[J]. 河北医学, 2020, 26(4): 563-568.
HUANG Cuimin, YE Xiuqun, LUO Yueji. Effects of Momordica Saponin on Proliferation Apoptosis and JAK / STAT5 Pathway of Human Malignant Melanoma Skmel-2 Cells. HeBei Med, 2020, 26(4): 563-568.
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2020, Vol. 26 
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