Abstract：Objective: To investigate the effect of procaine on proliferation and apoptosis of cervical cancer cells and its mechanism. Methods: SiHa cells were treated with procaine with final concentrations of 0.0 mm / L, 0.5 mm / L, 1 mm / L, and 2 mm / L. pcDNA and pcDNA trail were transfected into untreated SiHa cells respectively, which were recorded as pcDNA group and pcDNA trail group. After Si NC and Si trail were transfected into SiHa cells, they were treated with procaine with 2 mm / L, which were recorded as procaine 2 mm / L + Si NC group and procaine 2 mm / L + Si trail group, respectively The liposome method was used. MTT method was used to detect cell proliferation; flow cytometry was used to detect apoptosis; QRT PCR was used to detect trail mRNA expression; Western blot was used to detect protein expression. Results: Procaine can inhibit the expression of CyclinD1, Bcl-2, p21, Caspase-8 and Bax, inhibit the proliferation of SiHa, promote apoptosis and promote the expression of trail. Overexpression of trail inhibited the proliferation of SiHa and promoted apoptosis. Inhibition of TRAIL expression reversed the inhibition of proliferation and apoptosis of cervical cancer cell line SiHa by procaine. Conclusion: Procaine can inhibit the proliferation of SiHa and promote the apoptosis of cervical cancer cells, which may be related to the regulation of trail. It will provide new ideas and targets for the treatment of cervical cancer.
金翠红, 刘淑娟, 静金艳. 鲁卡因调节TRAIL基因抑制宫颈癌细胞增殖和诱导细胞凋亡的机制研究[J]. 河北医学, 2020, 26(1): 138-142.
JIN Cuihong, et al. Study on the Mechanism of Iucain Negulating TRAIL Gene to Inhibit the Proliferation and Induce Apoptosis of Cervical Cancer Cells. HeBei Med, 2020, 26(1): 138-142.
[1] 黄梦微,杜靖然.局部晚期宫颈癌的治疗现状与进展[J].现代妇产科进展,2018,27(9):75-77. [2] Li Y C, Wang Y, Li D D, et al. Procaine is a specific DNA methylation inhibitor with anti-tumor effect for human gastric cancer[J].Cell Biochem, 2017, 119(2):2440~2449. [3] Borutinskaite V, Bauraite-Akatova J, Navakauskiene R. Anti-leukemic activity of DNA methyltransferase inhibitor procaine targeted on human leukaemia cells[J]. Open Life Sci, 2016, 11(1):322~330. [4] 夏阳,李谦.肿瘤坏死因子相关诱导凋亡配体的临床应用[J].药物生物技术,2015,22(5):452~456. [5] 冷远景,陈捷,陆文宝,等.前列腺癌PC-3细胞抗TRAIL诱导凋亡的实验研究[J].泌尿外科杂志(电子版),2017,9(4):31~36. [6] 党晓东,来炳祺,何元.普鲁卡因抑制脂多糖诱导的结肠癌细胞肿瘤侵袭性的作用机制[J].局解手术学杂志,2018,27(8):7~10. [7] Li C, Gao S, Li X, et al. Procaine inhibits proliferation and migration of colon cancer cells through inactivation of the ERK/MAPK/FAK pathways by regulation of RhoA[J]. Oncol Res, 2017, 26(2):209~217. [8] 李金钱,徐宛婷,李皖豫,等.TRAIL抗癌作用的研究进展[J].现代肿瘤医学,2018,26(12):135~138. [9] 张文颖,袁海花,姜斌,等.TRAIL基因修饰的树突状细胞诱导黑色素瘤细胞凋亡的实验研究[J].现代免疫学,2017,37(5):390~394. [10] Hao Z, Han X, Sun X, et al. Fully human monoclonal antibodies to TRAIL-R1 enhance TRAIL-induced apoptosis via activation of caspase-8 pathway[J]. Biochem Biophys Res Commun, 2016,475(2):238~244. [11] Im J Y, Kim B K, Lee J Y, et al. DDIAS suppresses TRAIL-mediated apoptosis by inhibiting DISC formation and destabilizing caspase-8 in cancer cells[J]. Oncogene, 2018, 37(9):1251~1262. [12] Fang Y, Chen X, Bai Q, et al. IL‐9 inhibits HTB‐72 melanoma cell growth through upregulation of p21 and TRAIL[J].Surg Oncol, 2015, 111(8):969~974.
2020, Vol. 26 
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