Abstract：Objective: To study the protective effects of Ganoderma lucidum polysaccharides on adriamycin induced myocardial injury, and to explore the mechanism of adriamycin induced myocardial cell.Methods: Different concentrations of doxorubicin (1, 2, 4, 6μmol/L) with H9C2 were cultured for 6, 12, 24h,then calculated the cell inhibitory rates at different time and concentration;H9C2 cardiac myocytes were cultured with Ganoderma lucidum polysaccharides of different mass concentrations (0, 3.125, 6.25, 12.5, 25mg/L),adding doxorubicin after 1h,observing morphological changes of the cells after 24h,calculating the cell inhibition rate;MCF-7,KB,K562 were cultured with Ganoderma lucidum polysaccharides,adding doxorubicin after 1h,after 48h calculating the cell inhibition rate Results: The inhibition rate of 24h was the highest when 2μmol/L was cocultured with cells,25mg/L Ganoderma lucidum polysaccharides can significantly reduce the death of myocardial cells induced doxorubicin, and not reduce the anti-tumor effect of drugs. Conclusion: GL-PS has a protective effect on myocardial cells, had no effect on adriamycin inhibit the growth of tumor cells, there is no synergistic effect.
徐繁, 李潇, 刘兰芳, 潘兴华, 张力, 张圣林, 林萍萍, 李青山. 灵芝多糖对多柔比星诱导心肌细胞损伤保护作用的研究[J]. 河北医学, 2017, 23(10): 1588-1590.
XU Fan, LI Xiao, LIU Lanfang, et al. Study on the Protective Effect of Ganoderma Lucidum Polysaccharides| about Doxorubicin Inducing Myocardial Cell Injury. 河北医学, 2017, 23(10): 1588-1590.
[1]曹容华.灵芝多糖对免疫调节作用的研究[J].山东医科大学学报,1993,31(4):287~290. [2] Chen YG, Shen ZJ, Chen X. Modulatory effect of Ganoderma lucidum polysaccharides on serum antioxidant enzymes activities in ovarian cancer rats[J].Carbohydrate Polymers, 2009, 78(2): 258~262. [3] Minotti G,Menna P,Salvatorelli E,Cairo G Gianni L.Anthracyclines:molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity[J].Pharmacol Rev,2004,56:185~229. [4] Liang Z, Yi Y, Guo Y, et al. Chemical characterization and antitumor activities of polysaccharide extracted from Ganoderma lucidum[J].International Journal of Molecular Sciences, 2014, 15(5): 9103.
2017, Vol. 23 
冀公网安备13080202000677号