Abstract：Objective: To detect specific maternal serum protein markers for Down's syndrome early. Methods: Ten maternal sera (14~20 weeks) and 10 normal maternal sera at the same time point were collected. The differential expression proteins were analyzed by mass spectrometry and further verified by Western blot. Results: By mass spectrometry analysis, 13 proteins with abnormal expression of more than 1.0 times were found in maternal serum of Down syndrome fetuses. DAVID functional analysis showed that 13 differentially expressed proteins were mainly involved in lipoprotein biosynthesis and decomposition, apoptosis and platelet degranulation. The abnormal expression of FN1 and IGFBP3 in maternal serum of Down's syndrome fetus was 2.5 times higher than that in normal control group (P<0.05). Conclusion: Mass spectrometry is an effective method for screening maternal serum protein markers of Down syndrome, and FN1 and IGFBP3 may be potential new markers for screening Down syndrome fetuses.
肖艳平, 付久园, 葛永梅, 张金环. 基于质谱筛选唐氏综合症母体血清蛋白标志物的研究[J]. 河北医学, 2019, 25(12): 2082-2086.
XIAO Yanping, FU Jiuyuan, GE Yongmei, et al. Screening Maternal Serum Protein Markers of Down's Syndrome Based on Mass Spectrometry. HeBei Med, 2019, 25(12): 2082-2086.
[1] Park A H, et al.Identification of hearing loss in pediatric patients with down syndrome[J]. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2016,146(1):135~140. [2] Liu G, et al.Apolipoprotein E gene polymorphism and its effect on plasma lipids[J]. Medical Journal of Wuhan University, 2017,38(2):267~270. [3] Ruiz M, et al.High-density lipoprotein-associated apolipoprotein M limits endothelial inflammation by delivering sphingosine-1-phosphate to the sphingosine-1-phosphate receptor 1[J]. Arterioscler Thromb Vasc Biol, 2017,37(1):118~129. [4] Mariosa D, et al.Blood biomarkers of carbohydrate, lipid and apolipoprotein metabolisms and risk of amyotrophic lateral sclerosis: a more than 20 year follow-up of the swedish AMORIS cohort[J]. Annals of Neurology, 2017,81:718~728. [5] Wang Y A, et al.IGFBP3 modulates Lung tumorigenesis and cell growth through IGF1 signaling[J]. Molecular Cancer Research, 2017,15(7):896~904. [6] Xu J, et al.Overexpression of the Kininogen-1 inhibits proliferation and induces apoptosis of glioma cells[J]. Journal of Experimental & Clinical Cancer Research, 2018,37(1):180. [7] Ohtsubo H, et al.Identification of mutations in FN1 leading to glomerulopathy with fibronectin deposits[J]. Pediatric Nephrology, 2016,31(9):1459~1467. [8] Lee J C, et al.Characterization of FN1–FGFR1 and novel FN1–FGF1 fusion genes in a large series of phosphaturic mesenchymal tumors[J]. Mod Pathol, 2016,29(11):1335~1346. [9] 黄佳敏,等.血清胰岛素和IGFBP3水平与子宫内膜癌相关性研究[J]. 浙江预防医学, 2016,28(4):358~361.
2019, Vol. 25 
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