Abstract：Objective: To investigate the relationship between the acetylation level of Survivin protein and the expression of PCAF,P300 and the clinical features of esophageal carcinoma. Methods: The Survivin protein acetylation level were examined via co-immunoprecipitation experiment in 70 cases esophageal carcinoma tissue and peficancerous tissues. The PCAF and P300 protein were examined via Immunohistochemical staining experiment in esophageal carcinoma tissue.The relationship was analyzed between Survivin acetylation level and expression of PCAF, P300 and clinical case characteristics. Results: (1)The Survivin protein was acetylated in esophageal carcinoma tissue. The Survivin protein acetylation level in esophageal carcinoma tissue was 72.05±1.26%, it was obviously higher than the peficancerous tissues(13.03±1.34%). There was statistics difference (P<0.05);The Survivin protein acetylation level were closely related to esophageal carcinoma staging, differentiation and lymph node metastasis (P<0.05); (2) The positive expression of PCAF was 38.57% in cancer tissues,which was lower than that in normal tissues which was 87.14%. There was statistics difference (P<0.05); (3) The positive expression of P300 was 72.86%% in cancer tissues,which was higher than that in normal tissues which was 15.71%%. There was statistics difference (P<0.05); (4)The Survivin acetylation level and expression of PCAF in esophageal carcinoma was no correlation.The correlation coefficient r=0.2421 (P>0. 05);(5)The Survivin acetylation level and expression of P300 in esophageal carcinoma was interrelated.The correlation coefficient r=0.7453 (P<0. 05). Conclusions: Survivin protein acetylated in esophageal cancer tissue, and the occurrence and development of esophageal cancer may be closely related to the degree of acetylation of Survivin protein. The acetylation of Survivin protein was correlated with acetyltransferase P300, but not with PCAF.
[1] 王森舟,张弢,刘辉琦,等.靶向Bmi-l基因RNA干扰对食管癌细胞增殖、侵袭及顺铂敏感性的作用[J].实用医学杂志,2017,33(5):683~686. [2] 沈绪光,师磊,曲昌发.与食管癌相关的癌症干细胞膜标志物的研究进展[J].现代肿瘤学,2019,27(1):154~157. [3] Schuetze KB, M cKinsey TA, Long CS. Targeting cardiac fibroblasts to treat fibrosis of the heart:Focus on H DACs[J]. Journal of M olecular & Cellular Cardiology,2014,70:100~107. [4] 王云,李运红.胃癌中Survivin的研究进展[J].中国临床研究,2017,30(4):546~548. [5] 徐赛群,王伟宁,刘丽,等.Survivin、Livin和PTEN在结肠癌组织中的表达及意义[J].临床消化病杂志,2018,30(4):233~236. [6] Tomomi Sc, Shuhei Sk, Kenta Kt,et al.Olanzapine, an atypical antipsychotic, inhibits survivin expression and sensitizes cancer cells to chemotherapeutic agents[J].Anticancer research, 2017,37(11):6177~6188. [7] 张定富,吴秋芳,戈长征,等.survivin和caspase-3检测在鼻咽癌放疗敏感性检测中的意义[J].国际检验医学杂志,2017,38(24):3463~3465. [8] 陈恒屹.组蛋白去乙酰化酶抑制剂与肿瘤治疗研究进展[J].重庆医学,2017,46(30):4280~4282 [9] 吕斌娜,梁文星.蛋白质乙酰化修饰研究进展[J].生物技术通报,2015,31(4):166~174. [10] 邱卫芳,邱卫华.PCAF蛋白在人宫颈癌组织中的表达及其临床意义[J].宁夏医科大学学报,2014,36(10):1128~1129.
2019, Vol. 25 
冀公网安备13080202000677号