Abstract：Objective: To analyze the expression level and regulation mechanism of microRNA-23a (miR-23a), microRNA-24-2 (miR-24-2) and microRNA-27a (miR-27a) in patients with primary gouty arthritis (PGA). Methods: 50 patients with PGA in our hospital were selected as case group and 30 health physical examination personnel as control group. The expression levels of miR-23a, miR-24-2 and miR-27a were detected by real-time fluorescent quantitative PCR. Pearson correlation analysis was used to analyze the relationship between the expressions of miR-23a, miR-24-2 and miR-27a. The data were included in statistical analysis. Results: The plasma fasting blood glucose, uric acid, white blood cell count, neutrophils, triglycerides and apolipoprotein B100 in the case group were significantly higher than those in the control group (all P<0.05), apolipoprotein A1 was significantly lower than those in the control group (P<0.01), but there was no significant difference in total cholesterol between the two groups (P>0.05). Compared with the control group, the expression levels of miR-23a, miR-24-2 and miR-27a in the case group were significantly decreased (P<0.01). Pearson correlation analysis showed that there was a positive correlation between the expression levels of miR-23a, miR-24-2 and miR-27a in peripheral blood mononuclear cells of patients (P<0.01). Urate (100 g/ml) was used to stimulate peripheral blood mononuclear cells in healthy people. The results showed that the expression levels of miR-23a, miR-24-2 and miR-27a in peripheral blood mononuclear cells in the stimulation group were significantly lower than those in the non-stimulation group (P<0.01). Conclusion: Mi-23a, mir-24-2 and mir-27a may play an important role in the inflammatory immune response of PGA as negative inflammatory regulators, and mir-24-2 may regulate the lipoprotein of PGA.
李宁宁, 戴冰冰, 雷蕾, 张昊, 刘畅, 张金涛, 金香花, 田丽, 滕小铭. 原发性痛风性关节炎患者miR-23a miR-24-2和miR-27a的表达水平及调节机制[J]. 河北医学, 2019, 25(8): 1233-1236.
LI Ningning, DAI Bingbing, LEI Lei, et al. The Expression Level and Regulation Mechanism of MicroRNA-23a MicroRNA-24-2 and MicroRNA-27a in Patients with Primary Gouty Arthritis. HeBei Med, 2019, 25(8): 1233-1236.
[1] 朱新,许艳春.X线CT及MRI在痛风性关节炎诊断中的价值[J].河北医学,2015,21(10):1600~1601. [2] Mohr AM, Mott JL.Overview of microRNA biology[J].Semin Liver Dis,2015,35(1):3~11. [3] Zhang H, Huang X, Ye L, et al. B cell-related circulating microRNAs with the potential value of biomarkers in the differential diagnosis, and distinguishment between the disease activity and lupus nephritis for systemic lupus erythematosus[J].Front Immunol,2018,9(1):1473. [4] Angiolilli C, Marut W, Van der Kroef M,et al. New insights into the genetics and epigenetics of systemic sclerosis[J].Nat Rev Rheumatol,2018,14(11):657~673. [5] Wang Y, Xu D, Wang B, et al.Could microRNAs be rgulators of gout rathogenesis[J].Cell Physiol Biochem,2015,36(6):2085~2092. [6] 刘艳霞,张璐玉,崔艳艳,等.miRNA在食管鳞癌发病和治疗中的作用机制[J].郑州大学学报(医学版),2018,53(2):137~142. [7] 黄耀孟,吴珺.miRNA在乳腺癌诊断、预后和预测中的应用[J].河北医科大学学报,2018,39(9):1100~1106. [8] Estevez-Garcia IO, Gallegos-Nava S, Vera-Perez E, et al.Levels of cytokines and microRNAs in individuals with asymptomatic hyperuricemia and ultrasonographic findings of gout: A bench-to-bedside approach[J].Arthritis Care Res (Hoboken),2018,70(12):1814~1821. [9] Hua K, Chen YT, Chen CF, et al.MicroRNA-23a/27a/24-2 cluster promotes gastric cancer cell proliferation synergistically[J].Oncol Lett,2018,16(2):2319~2325. [10] Su R, Dong L, Zou D, et al.microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia[J].Oncogene,2016,35(46):6001~6014. [11] Han Q, Bing W, Di Y, et al.Kinsenoside screening with a microfluidic chip attenuates gouty arthritis through inactivating NF-κB signaling in macrophages and protecting endothelial cells[J].Cell Death Dis,2016,7(9):e2350. [12] Li X, Liu X, Xu W, et al. c-MYC-regulated miR-23a/24-2/27a cluster promotes mammary carcinoma cell invasion and hepatic metastasis by targeting Sprouty2[J].Biol Chem,2013,288(25):18121~18133. [13] Okur MN, Russo A, O'Bryan JP.Receptor tyrosine kinase ubiquitylation involves the dynamic regulation of Cbl-Spry2 by intersectin 1 and the Shp2 tyrosine phosphatase[J].Mol Cell Biol,2014,34(2):271~279. [14] Park JW, Wollmann G, Urbiola C, et al. Sprouty2 enhances the tumorigenic potential of glioblastoma cells[J].Neuro Oncol,2018,20(8):1044~1054.
2019, Vol. 25 
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