Abstract：Objective: To establish and apply the Methods of LAMP assay for the detection of Shigella of infectious diarrhea patients from the enteric clinicsin. Methods: The specific primers of the conserved regions of Shigella by using Primer Explorer V5 online and composed to establish the LAMP assay for detection of Salmonella. The 760 fecal specimens among diarrhea patients visiting the enteric clinicsin in Hospital of Chengde Medical College from 2016 was collected and test by Real-time PCR ,LAMP and the Traditional Method. The specificity and sensibility of LAMP were evaluated by comparing with Real-time PCR and the Traditional. Results: There was no statistical difference in the specificity of 3 method(P＞0.05). LAMP showed fine specificity and high sensitivity. Conclusion: LAMP assay was performed in water oven and the results could be directly determined by eyes under visible light or UV lamp. Because of its rapidity and minimal equipment requirement,LAMP assay for detection of Shigella in primary diseases control and prevention agency and primary health institutions.
王伟, 张瑞莲, 玄立印, 王海连. 环介导技术在细菌性痢疾检测中的应用[J]. 河北医学, 2017, 23(9): 1496-1497.
WANG Wei, et al. The Application of LAMP Technique in Detection of the Bacillary Dysentery. 河北医学, 2017, 23(9): 1496-1497.
2017, Vol. 23 
冀公网安备13080202000677号