Abstract：Objective: To study the effect and mechanism of shikonin on apoptosis of SMMC-7721 cells of liver cancer. Methods: Different concentration of shikonin were used to interfere with liver cancer SMMC - 7721 cells, determined by MTT method to observe the cell proliferation inhibition, and using flow cytometry to test the apoptosis rate after 24h, and further using PCR and Western blot to detect the mRNA and protein expression of caspase3 and caspase9. Results: the growth of liver cancer SMMC-7721 cells was inhibited significantly at 24h, 48h and 72h by shikonin (0, 0.5, 1.0, 2.0, 4.0μg/mL), indicating the dual dependence of time and concentration. According to the results of flow cytometry detection, the apoptosis rate of human liver cancer SMMC-7721 cells was significantly higher than that of the control group after 24 h of action of different concentrations of shikonin (5, 10, 20μg/mL), and the apoptosis rate was significantly increased with the increase of shikonin concentration. In addition, the increased concentration of shikonin can promote the expression of mRNA and protein of caspase3 and caspase9 in human liver cancer SMMC-7721 cells. Conclusion: Shikonin can inhibit the apoptosis of SMMC-7721 cells in liver cancer by promoting the mRNA and protein expression of caspase3 and caspase9.
张淑玲,梁兰,邹冶,鲁艳杰. 紫草素对SMMC-7721肝癌细胞凋亡的影响[J]. 河北医学, 2018, 24(12): 2012-2015.
ZHANG Shuling, LIANG Lan, ZOU Ye, et al. Effects of Shikonin on cell apoptosis of human liver cancer cell line SMMC-7721. HeBei Med, 2018, 24(12): 2012-2015.
[1] 李秋林,余家华,容敏华,等.2013年广西肿瘤登记地区肝癌发病与死亡分析[J].中国癌症防治杂志,2017,9(6):462~468. [2] Bo Tang, Guangying Qi, Xiaoyu Sun, et al.HOXA7 plays a critical role in metastasis of liver cancer associated with activation of Snail [J].moL Cancer, 2016: 15(1): 57. [3] Jeong Hee Yoon, Joong-Won Park, Jeong Min Lee, et al.Noninvasive diagnosis of hepatocellular carcinoma: elaboration on korean liver cancer study group-national cancer center korea practice guidelines compared with other guidelines and remaining issues [J]. Korean Radiol , 2016:17(1): 7~24. [4] 张萍.紫草素对荷肝癌裸小鼠移植瘤生长的影响及其机制研究[J].中医药学报,2017,45(6):45~49. [5] 赵雪梅,王桂玲,费洪荣,等.紫草有效成分的提取及其抗炎作用研究[J].中药药理与临床,2008,24(4):36~38. [6] 代巧妹,云雨,沈宁,等.紫草素提取、合成及测定方法研究进展[J].中医药信息,2014,31(1):109~111. [7] Feng-Ying Zhang, Yi Hu, Zhong-You Que, et al.Shikonin inhibits the migration and invasion of human glioblastoma cells by targeting phosphorylated β-catenin and phosphorylated PI3K/Akt: a potential mechanism for the anti-glioma efficacy of a traditional Chinese herbal medicine[J]. Int moL Sci,2015,16(10): 23823~23848. [8] Chuanjiang Huang, Yinan Luo, Jingwei Zhao, et al.Shikonin kills glioma cells through necroptosis mediated by RIP-1[J]. PLoS One,2013,8(6): e66326. [9] Hao Wu, Jiansheng Xie, Qiangrong Pan, et al.Anticancer agent shikonin is an incompetent inducer of cancer drug resistance[J]. PLoS One,2013,8(1): e52706. [10] Phillipps HR,Hurst PR.XIAP:a potential determinant of ovarian follicular fate[J].Reproduction,2012,144(2):165~176. [11] Park HH.Structural features of caspase-activating complexes[J].Int moL Sci,2012,13(4):4807~4818. [12] Liu J,Shu Y,Zhang Q,et al.Dihydromyricetin induces apoptosis and inhibits proliferation in hepatocellular carcinoma cells[J].Oncol Lett, 2014,8(4):1645~1651. [13] Kao SJ,Lee WJ,Chang JH,et al.Suppression of reactive oxygen species‐mediated ERK and JNK activation sensitizes dihydromyricetin‐induced mitochondrial apoptosis in human non‐small cell lung cancer[J]. Environ Toxicol,2017,32(4):1426~1438. [14] Sun Y,Wang C,Meng Q,et al.Targeting P-glycoprotein and SORCIN:Dihydromyricetin strengthens anti-proliferative efficiency of adriamycin via MAPK/ERK and Ca2+-mediated apoptosis pathways in MCF-7/ADR and K562/ADR[J].Cell Physiol,2018,233(4):3066~3079.
2018, Vol. 24 
冀公网安备13080202000677号