Abstract：Objective: To analyze the effect of sinomenine (SIN) combined with epirubicin (EPI) on the changes of invasion-related proteins in melanoma cells. Methods: The invasion status of melanoma A375 cells under different conditions was detected by Transwell. They were divided into control group (given normal saline), SIN group (10 g/mL, 100 g/mL as low concentration and high concentration), EPI group (5 g/mL, 50 g/mL as low concentration and high concentration), SIN+EPI group (10 g/mL, 5 g/mL) and SIN+EPI group (5 g/mL). The expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) proteins and their mRNA were detected by RT-PCR and Western blot. Results: The inhibition rate of SIN+EPI group on the invasion of melanoma A375 cells was significantly higher than that in group SIN and EPI (P<0.05). Moreover, with the increase of SIN and EPI concentration, the inhibition of SIN and EPI on the invasion of A375 cells increased significantly. Compared with the control group, the levels of MMP-2, MMP-9 protein and mRNA in SIN group, the EPI group and the SIN+EPI group were inhibited in varying degrees. Among them, the inhibition effect of SIN+EPI group was higher (P<0.05). Conclusion: The use of low concentration (10μg/mL) SIN can effectively promote the inhibitory effect of low concentration of EPI on the invasion of melanoma cells, and SIN can be used as an important drug for adjuvant chemotherapy. It can play a role in improving the clinical efficacy. SIN combined with EPI chemotherapy drugs can play a synergistic and synergistic effect, and can reduce the dosage of EPI, in order to prevent a series of toxic and side effects caused by high concentration of chemotherapeutic drugs, so it has good safety and effectiveness.
王妍,李纯诚,王月清. 青藤碱与表柔比星联合处理对黑色素瘤细胞侵袭相关蛋白变化的影响[J]. 河北医学, 2018, 24(12): 2009-2011.
WANG Yan, et al. Effects of Sinomenine combined with Epirubicin on the Changes of Invasion Related Proteins in Melanoma Cells. HeBei Med, 2018, 24(12): 2009-2011.
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2018, Vol. 24 
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