Abstract：Objective: To measure the expression level of DNA methyltransferase 1 (DNMT1) and the methylation level of trefoil factor 1 (TFF1) in lung adenocarcinoma,and to analyze their clinical significances. Methods: The methylation level of TFF1 in human lung adenocarcinoma tissues was detected by pyrosequencing.The mRNA levels of TFF1 and DNMT1 were detected by reverse transcription coupled to the quantitative polymerase chain reaction (RT-qPCR).Their protein levels were detected by immunohistochemical staining and Western blot.The correlation of the methylation level of TFF1 and expression level of DNMT1 with pathological characteristics of lung adenocarcinoma was explored. Results: The methylation rate of the TFF1 gene was significantly higher in the lung adenocarcinoma tissues compared with that of paracancerous tissues (P<0.05).PCR results showed that the mRNA level of TFF1 was significantly lower in the lung adenocarcinoma tissues,while that of DNMT1 was significantly higher than those of paracancerous tissues (P<0.05).Immunohistochemistry showed a significantly lower protein level of TFF1,but higher protein level of DNMT1 in the lung adenocarcinoma tissues than those of paracancerous tissues (P<0.05).Western blot results showed a significantly lower protein level of TFF1,but higher protein level of DNMT1 in the lung adenocarcinoma tissues than those of paracancerous tissues (P<0.05).TFF1 methylation level and DNMT1 expression were significantly correlated with the TNM staging and lymph node metastasis of lung adenocarcinoma (P<0.05).The statistical analysis showed that TFF1 methylation and DNMT1 protein expression were related in lung adenocarcinoma tissues, it was positive correlation (r=0.538, P<0.05). Conclusion: During the cancerous progression of lung adenocarcinoma,DNMT1 is closely linked with methylation of the TFF1 gene.High level of DNMT1 and high methylation level of TFF1 both promote the occurrence and development of lung adenocarcinoma.
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2025, Vol. 31 
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