Abstract：Objective: To investigate the association between the expression levels of microRNA-1470 (miR-1470), POZ domain and AT-binding motif zinc finger protein 1 (PATZ1) in hepatocellular carcinoma (HCC) tissues and patient prognosis within 5 years. Methods: A total of 135 patients with HCC who underwent radical resection at Handan Central Hospital from September 2015 to August 2018 were enrolled in this study. HCC tissues and corresponding adjacent normal tissues (normal liver tissues more than 2 cm away from the tumor margin) were collected during surgery. Immunohistochemistry was used to detect PATZ1 protein expression; real-time fluorescence quantitative PCR was used to detect miR-1470 and PATZ1 mRNA expression. Patients were followed up for 5 years, and 5-year overall survival rates were calculated. The expression of miR-1470 and PATZ1 mRNA and protein in HCC tissues and adjacent normal tissues was compared; the relationship between miR-1470 and PATZ1 mRNA expression and clinicopathological parameters, their correlation, and their association with prognosis were analyzed; and the risk factors affecting prognosis were identified. Dual luciferase reporter gene assay was used to verify the target relationship between miR-1470 and PATZ1. Results: The positive expression rate of PATZ1 protein in HCC tissues was significantly lower than that in adjacent normal tissues (P<0.05). Compared with adjacent normal tissues, the expression level of miR-1470 was significantly upregulated and the expression level of PATZ1 mRNA was significantly downregulated in HCC tissues (P<0.05). Patients with low miR-1470 and high PATZ1 mRNA expression in HCC tissues had significant differences in serum alpha-fetoprotein (AFP) level, China Liver Cancer Staging System (CNLC) stage, and the presence or absence of vascular tumor thrombus (P<0.05). The expression of miR-1470 and PATZ1 mRNA was negatively correlated in HCC tissues (P<0.05). The 5-year overall survival rates of patients with low miR-1470 expression and high PATZ1 mRNA expression were significantly higher than those of patients with high miR-1470 expression and low PATZ1 mRNA expression (P<0.05). CNLC stage III, vascular tumor thrombus, high miR-1470 expression, and low PATZ1 mRNA expression were independent risk factors for poor prognosis in HCC patients (P<0.05). miR-1470 could negatively regulate PATZ1 expression. Conclusion: miR-1470 is upregulated and PATZ1 is downregulated in HCC tissues, and the two are negatively correlated. Both can be used as biomarkers to evaluate the prognosis of HCC patients, and miR-1470 can negatively regulate PATZ1 expression.
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2024, Vol. 30 
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