miR-1297靶向HMGA1基因以调节胃癌细胞的侵裘和迁移

doi:10.3969/j.issn.1006-6233.2024.05.02

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摘要 目的: 探讨miR-1297是否可以通过靶向高迁移率组A1(HMGA1)调控胃癌的迁移和侵袭。方法: 通过逆转录定量聚合酶链反应(RT-qPCR)测定了胃癌组织(n=30)和邻近正常组织中miR-1297和HMGA1的表达水平。体外培养胃腺癌SGC-7901细胞系和人正常胃粘膜上皮细胞GES-1。将SGC-7901细胞分为空白组、阴性对照组、miR-1297 mimics组、miR-1297 inhibitor和miR-1297 inhibitor+HMGA1 siRNA组。采用Transwell法检测SGC-7901细胞的迁移和侵袭情况。Western blot检测蛋白水平的变化。荧光素酶报告基因法检测miR-1297特异性靶基因。结果: miR-1297在胃癌组织和SGC-7901细胞中表达下调,HMGA1 mRNA水平同时升高(P<0.001),并且miR-1297与HMGA1 mRNA表达均呈负相关关系(P<0.05)。与空白组和阴性对照组相比,miR-1297 mimics组可抑制细胞在体外的迁移和侵袭能力(P<0.05)。此外,miR-1297通过靶向HMGA1的3'-UTR下调HMGA1。与空白和阴性对照相比,转染miR-1297 inhibitor下调miR-1297表达后细胞迁移和侵袭能力显著增加(P<0.05),同时转染HMGA1 siRNA(miR-1297 inhibitor+HMGA1 siRNA组)后这种促进能力被逆转(P<0.05)。结论: miR-1297通过靶向HMGA1抑制SGC-7901细胞的迁移和侵袭,提示miR-1297/HMGA1轴为胃癌提供了一种新的前瞻性治疗策略。

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关键词 ：胃癌, HMGA1, 侵袭, 迁移, miR-1297

Abstract：Objective: To investigate whether miR-1297 regulates the migration and invasion of gastric cancer by targeting high mobility group A1 (HMGA1). Methods: The expression levels of miR-1297 and HMGA1 in gastric cancer (n=30) and adjacent normal tissues were determined by RT-qPCR. Gastric adenocarcinoma SGC-7901 cell line and human normal gastric mucosal epithelial cells GES-1 were cultured in vitro. SGC-7901 cells were divided into blank group, negative control group, miR-1297 mimics group, miR-1297 inhibitor and miR-1297 inhibitor+HMGA1 siRNA group. Transwell method was used to detect the migration and invasion of SGC-7901 cells. Western blot tests for changes in protein levels. The specific target genes of miR-1297 were detected by luciferase reporter gene assay. Results: The expression of miR-1297 was down-regulated in gastric cancer tissues and SGC-7901 cells, and HMGA1 mRNA level was increased at the same time (P<0.001), and miR-1297 and HMGA1 mRNA expression were negatively correlated (P<0.05). Compared with blank group and negative control group, miR-1297 mimics group could inhibit the migration and invasion ability of cells in vitro (P<0.05). In addition, miR-1297 down-regulates HMGA1 by targeting its 3'-UTR. Compared with blank and negative controls, transfection with miR-1297 inhibitor after downregulation of miR-1297 expression significantly increased cell migration and invasion ability (P<0.05). At the same time, the promoting ability was reversed after transfection of HMGA1 siRNA (miR-1297 inhibitor+HMGA1 siRNA group) (P<0.05). Conclusion: miR-1297 inhibits the migration and invasion of SGC-7901 cells by targeting HMGA1, suggesting that miR-1297/HMGA1 axis provides a new prospective therapeutic strategy for gastric cancer.

Key words： Gastric cancer HMGA1 Invasion Migration miR-1297

基金资助:河北省医学科学研究重点课题计划,(编号:20201396)

通讯作者: 李明

引用本文:

王英新, 马亚帅, 李忠, 王利伟, 张振铎, 李明. miR-1297靶向HMGA1基因以调节胃癌细胞的侵裘和迁移[J]. 河北医学, 2024, 30(5): 710-714.
WANG Yingxin, MA Yashuai, LI Zhong, et al. Effect of miR-1297 on Regulating Invasion and Migration of Gastric Cancer Cells via HMGA1 Gene. HeBei Med, 2024, 30(5): 710-714.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2024.05.02 或 http://www.hbyxzzs.cn/CN/Y2024/V30/I5/710

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