Abstract：Objective: To examine the M. tuberculosis strain diversity and describe the frequency and molecular mechanisms of resistance against first-line TB drugs in TB/HBV co-infected patients. Methods: A total of 100 strains of Mycobacterium tuberculosis from TB/HBV co-infected patients were analyzed retrospectively, among which Beijing strain was the most common (49%), followed by EAI strain (35%), and a few strains included Haarlem(2.0%), LAM(1.0%), MANU(3.0%) and T(4.0%). All strains were isolated from sputum and pleural effusion samples of patients from 2014 to 2018. Drug susceptibility testing, spoligotyping and 24-locus Mycobacterial Interspersed Repetitive Unit (MIRU-24 typing) were performed, and the rpoB, katG, inhA and inhA promoter, rpsL, rrs and embB genes were sequenced in all drug resistant isolates identified. Results: In total, 42/100 (42.0%) strains showed drug resistance; 9 (9.0%) were multi-drug resistant (MDR). Resistance rates were 25.0% (25/100) to isoniazid, 10.0% (10/100) to rifampicin, 5.0% (5/100) to ethambutol, and 40.0% (40/100) to streptomycin. Among rifampicin-resistant strains, 90% (9/10) of the isolates with rifampicin resistance had mutations in the RRDR (codons 507-533). Among isoniazid-resistant strains, mutations were found in 100% and 21% of katG and inhA (including the inhA promoter), respectively; their multidrug resistance was mainly associated with the Ser315Thr mutation in the katG gene (7/9, 77.8%). 59.5% of the strains with resistance belonged to the Beijing spectrum, and 66.7% of the MDR strains belonged to the Beijing spectrum. Conclusion: Nearly half (42%) of M. tuberculosis isolates from TB/HBV co-infected patients are drug-resistant strains, among which Beijing lineage is the main strain.
孟颖, 张倩, 支力强. 乙肝合并结核分枝杆菌感染患者的结核耐药基因分布情况[J]. 河北医学, 2024, 30(2): 244-250.
MENG Ying, et al. Distribution of Tuberculosis Drug Resistance Genes in Patients with Hepatitis B Complicated with Mycobacterium Tuberculosis Infection. HeBei Med, 2024, 30(2): 244-250.
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2024, Vol. 30 
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