Abstract：Objective: To explore the molecular regulation mechanism of dental pulp stem cells. Methods: Human dental pulp stem cells (DPSC) were purchased and transfected with si-NC/SFRP1. The mRNA and protein expression level of SFRP1 was detected by qRT-PCR and Western blot. CCK-8 and flow cytometry were used to detect the proliferation activity and apoptosis of cells in each group. Cell wound healing and angiogenesis assays were used to detect the changes of cell migration and angiogenesis ability in each group. Western blot was used to detect the protein changes of p-p38 and p38 in MAPK signaling pathway. Results: After knocking down the expression of SFRP1 in DPSC, the proliferation activity of DPSC decreased and the apoptosis rate increased significantly. Cell migration and angiogenesis ability decreased significantly; the protein content of p-p38 in MAPK signaling pathway was significantly decreased. Conclusion: Knockdown of SFRP1 inhibited the proliferation and angiogenesis of dental pulp stem cells and promoted their apoptosis by inactivating the MAPK signaling pathway.
车艺蕾, 方璘. SFRP1通过MAPK信号通路对牙髓干细胞活性及血管生成的机制研究[J]. 河北医学, 2023, 29(8): 1259-1262.
CHE Yilei, FANG Lin. The Mechanism of SFRP1 on the Activity and Angiogenesis of Dental Pulp Stem Cells through MAPK Signaling Pathway. HeBei Med, 2023, 29(8): 1259-1262.
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2023, Vol. 29 
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