Abstract：Objective: To analyze the effects of microRNA-105-5p (miR-105-5p) on the proliferation and migration of glioma cells by regulating cell division cycle 5-like protein (CDC5L). Methods: Samples of 35 surgically treated human glioma tissues and normal brain tissues undergoing decompression due to traumatic brain injury were collected from our hospital. Logarithmic growth phase U251 cells were divided into control (control) group, transfected miR-105-5p negative control (miR-NC) group, transfected miR-105-5p mimics (miR-105-5p mimics) group, transfected silent CDC5L negative control (si-NC) group, transfected silent CDC5L (si-CDC5L) group , co-transfected miR-105-5p mimics and pcDNA (pcDNA) group, and co-transfected miR-105-5p mimics and pcDNA-CDC5L (miR-105-5p mimics+pcDNA-CDC5L) group. qRT-PCR was performed to measure the expression of miR-105-5p and CDC5L mRNA in glioma tissue, normal brain tissue, and U251 cells in each group; CCK-8 method was performed to measure cell proliferation; Transwell was performed to measure cell migration; Western blot method was performed to measure the expression of CDC5L, CyclinDl, matrix metalloproteinase 2 (MMP-2), MMP-9 and cyclin-dependent kinase inhibitor 1A (p21); dual luciferase reporter assay validated the targeting of miR-105-5p to CDC5L Results: Compared with normal brain tissue, the level of miR-105-5p in glioma tissue was significantly decreased,and CDC5L mRNA was significantly increased; miR-105-5p was negatively correlated with CDC5L (r= -0.512,P=0.002). Compared with the control group and si-NC group, the expression of p21 protein was increased in the si-CDC5L group (P<0.05), and the OD values at 24h, 48h and 72h, the number of migrating cells, the expression of CDC5L mRNA and the expression of CDC5L, CyclinDl, MMP-2 and MMP-9 proteins were decreased (P<0.05). Compared with the control group and miR-NC group, the expression of miR-105-5p and p21 protein was increased in the miR-105-5p mimics group (P<0.05), and the OD values at 24h, 48h and 72h, the number of migrating cells, the expression of CDC5L mRNA and the expression of CDC5L, CyclinDl, MMP-2 and MMP-9 proteins were decreased (P<0.05). Overexpression of CDC5L was able to reverse the effects of miR-105-5p on the proliferation and migration of U251 cells and CyclinDl, MMP-2, MMP-9, and p21 proteins (P<0.05); the dual luciferase experiment verified that CDC5L was the downstream target of miR-105-5p. Conclusion: miR-105-5p inhibits the proliferation and migration of glioma cells by down-regulating CDC5L.
宋海龙, 徐东为, 余秋男, 荆彦平, 李世冲, 刘建中, 刘京让. miR-105-5p调控CDC5L表达对胶质瘤细胞增殖迁移的影响[J]. 河北医学, 2023, 29(6): 906-911.
SONG Hailong, XU Dongwei, YU Qiunan, et al. Effects of miR-105-5p Regulating the Expression of CDC5L on Proliferation and Migration of Glioma Cells. HeBei Med, 2023, 29(6): 906-911.
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2023, Vol. 29 
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