Abstract：Objective: To investigate the effects of microRNA (miR)-30A-3p on migration and angiogenic phenotype of renal cell carcinoma (RCC) cells. Methods: The expression characteristics of miR-30a-3p in RCC cells were analyzed based on the expression data of miR-30a-3p in the Cancer Genome Atlas (TCGA) database. Predict the binding of miR-30a-3p to the 3'untranslated region (3' -UTR) of B cell activicting factor (BAFF) by bioinformatics websites. RCC cell lines ACHN and 786O were divided into LV-miR-30a-3p group [infected with lentiviral vector overexpressing miR-30a-3p], LV-NC group [infected with lentiviral vector negative control], LV-miR-30a-3p+pcDNA-BAFF group [co-infected with LV-miR-30a-3p and LV-Mir-30a-3p Overexpressing BAFF vector (pcDNA-BAFF)] and LV-miR-30a-3p+pcDNA-EV group [co-infected with LV-miR-30a-3p and empty vector (pcDNA-EV)]. Transwell migration chamber assay was used to detect cell migration ability, and tubule formation assay was used to detect cell angiogenesis ability. The expression of miR-30a-3p was detected by qPCR. The protein expressions of BAFF and vascular endothelial growth factor (VEGFA) were detected by Western blot. Targeting regulation of miR-30a-3p against BAFF in ACHN cells was detected by dual luciferase gene reporting assay. Results: Analysis of the data in the TCGA database showed that miR-30a-3p expression was significantly lower in the Cancer group compared to the Normal group (P<0.05). Compared with HK-2 cells, the expression of miR-30a-3p in ACHN and 786O cell lines was significantly decreased (both P<0.05). Compared with the LV-NC group, the expression of miR-30a-3p was increased in the LV-miR-30a-3p group (all P<0.05), while the cell mobility, tubule formation rate, and the expressions of BAFF and VEGFA were down-regulated in the LV-miR-30a-3p group (all P<0.05). According to bioinformatics prediction and double luciferase reporter assay, BAFF was found to be the target gene of miR-30a-3p. Compared with the LV-miR-30a-3p+pcDNA-EV group, cell mobility, tubule formation rate and BAFF expression in the LV-miR-30a-3p+pcDNA-BAFF group were up-regulated (all P<0.05). Conclusion: miR-30a-3p inhibits the migration and angiogenesis of RCC cells by targeting inhibits BAFF.
威力江·赛买提, 马军, 王玉杰. miR-30a-3p通过靶向调控BAFF在肾癌细胞迁移和血管生成能力中的作用研究[J]. 河北医学, 2023, 29(4): 529-535.
WEILIJIANG·Saimaiti, MA Jun, WANG Yujie. The Role of miR-30a-3p in the Migration and Angiogenesis Ability of Renal Cancer Cells through Targeted Regulation of BAFF. HeBei Med, 2023, 29(4): 529-535.
[1] Samimi-Dehkordi N,Taheri G,Afzali S,et al.Co-expression network analysis for renal cell carcinoma genes and in vitro confirmation of their expression in cell model in the presence of curcumin[J].Gene Rep,2022,26(1):101525-101534. [2] Roberto M,Botticelli A,Panebianco M,et al.Metastatic renal cell carcinoma management:from molecular mechanism to clinical practice[J].Front Oncol,2021,11(1):1-10. [3] Salgia N J,Dara Y,Bergerot P,et al.The changing landscape of management of metastatic renal cell carcinoma:current treatment options and future directions[J].Curr Treat Options Oncol,2019,20(5):1-8. [4] Argentiero A,Solimando A G,Krebs M,et al.Anti-angiogenesis and immunotherapy:novel paradigms to envision tailored approaches in renal cell-carcinoma[J].Clin Med,2020,9(5):1594-1603. [5] Hwang T I,Chen P C,Tsai T F,et al.Hsa-miR-30a-3p overcomes the acquired protective autophagy of bladder cancer in chemotherapy and suppresses tumor growth and muscle invasion[J].Cell Death Dis,2022,13(4):1-10. [6] Warakomska M,Tynecka M,Lemancewicz D,et al.The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness[J].Oncol Lett,2021,22(4):1-8. [7] Hayder H,O’Brien J,Nadeem U,et al.MicroRNAs:crucial regulators of placental development[J].Reproduction,2018,155(6):259-271. [8] 卢沐,付文博,宋亚芹,等.miR-30a-3p对食管鳞癌ECA-109细胞侵袭和增殖能力的影响及生物信息学分析[J].石河子大学学报:自然科学版,2017,35(4):462-467. [9] 林华山.miR-30a-3p 在肝癌中的表达功能及机制研究[D].浙江大学,2013. [10] Wang H,Kanmangne D,Li R,et al.miR-30a-3p suppresses the proliferation and migration of lung adenocarcinoma cells by downregulating CNPY2[J].Oncol Rep,2020,43(2):646-654. [11] Wang Y,Wang L,Yu X,et al.MiR-30a-3p targeting FLT1 modulates trophoblast cell proliferation in the pathogenesis of preeclampsia[J].Horm Metab Res,2022,54(9):633-640. [12] Lin H,Hong Y,Huang B,et al.Vimentin overexpressions induced by cell hypoxia promote vasculogenic mimicry by renal cell carcinoma cells[J].Biomed Res Int,2019,2019(1):7259691. [13] Bolkun L,Grubczak K,Schneider G,et al.Involvement of BAFF and APRIL in resistance to apoptosis of acute myeloid leukemia[J].Cancer,2016,7(14):1979. [14] Pelekanou V,Notas G,Athanasouli P,et al.APRIL and BAFF increase breast cancer cell stemness[J].bioRxiv,2017(1):1-12. [15] Bolkun L,Lemancewicz D,Jablonska E,et al.BAFF and APRIL as TNF superfamily molecules and angiogenesis parallel progression of human multiple myeloma[J].Ann Hematol,2014,93(4):635-644.
2023, Vol. 29 
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