Abstract：Objective: To explore the role and related mechanism of miR-653 in the maintenance of stemness of lung cancer stem cells based on the miRNA regulatory network. Methods: miRNA-seq was used to detect tumor tissues and adjacent tissues of lung cancer patients, and the regulatory network was drawn according to the difference of miRNA, and the target genes were analyzed by KEGG analysis. miR-NC, miR-653, oe-NC, and oe-MYC were transfected into the H460 lung cancer cell line by liposome transfection method. The cells were divided into the miR-NC group, miR-653 group, miR-653+oe-NC group, and miR-653+oe-MYC group. The expression of miR-653 and MYC were detected by qRT-PCR. The protein expression of CD133 and CD24 was detected by flow cytometry. CCK8 was used to detect cell proliferation. The proportion of pellet formation was detected by the limited dilution method. The targeting of miR-653 and MYC was detected by a luciferase reporter assay kit. Results: Compared with adjacent tissues, 245miRNAs were up-regulated and 363 miRNAs were down-regulated in tumor tissues, among which miR-653 was significantly down-regulated (P<0.05). The regulatory network predicted that miR-653 targeted 22 miRNAs and was involved in signaling pathways such as cell stemness maintenance. The fluorescence intensity of the miR-653 group was lower than that of the miR -NC group in wild-type MYC (P<0.05). Compared with the miR-NC group, the expression of miR-653 was increased and the expression of MYC was decreased in the miR-653 group. The protein expression of CD133 and CD24 was decreased, and the proportion of cell proliferation and pellet formation ratio was decreased (P<0.05). Compared with the miR-653+oe-NC group, the expression of MYC in the miR-653+oe-MYC group was increased. The protein expression of CD133, and CD24 was increased, and cell proliferation and pellet formation ratio was increased (P<0.05). Conclusion: The miRNA regulatory network is involved in the stemness maintenance of lung cancer cells, and miR-653 regulates MYC to inhibit the maintenance of lung cancer cell stem cells.
何达, 马子越, 席俊峰. 基于miRNA调控网络探究miR-653在肺癌干细胞干性维持中的作用[J]. 河北医学, 2023, 29(2): 226-232.
HE Da, MA Ziyue, XI Junfeng. Probing the Role of miR-653 in Stemness Maintenance of Lung Cancer Stem Cells Based on miRNA Regulatory Network. HeBei Med, 2023, 29(2): 226-232.
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2023, Vol. 29 
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