LINC00342调控miR-384对肺鳞癌细胞增殖侵袭迁移的影响研究

doi:10.3969/j.issn.1006-6233.2023.02.01

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摘要 目的: 探讨LINC00342通过miR-384对肺鳞癌细胞增殖、侵袭、迁移的影响。方法: 将肺鳞癌SK-MES-1细胞分为:ctrl组(正常培养的SK-MES-1细胞)、si-NC组(转染si-NC)、si-LINC00342组(转染si-LINC00342)、si-LINC00342+inhibitor-NC组(si-LINC00342和inhibitor-NC共转染)、si-LINC00342+miR-384 inhibitor组(si-LINC00342和miR-384 inhibitor共转染);RT-qPCR检测细胞中LINC00342、miR-384表达;CCK-8法及Transwell小室法分别检测细胞增殖和侵袭;划痕实验及流式细胞仪分别检测细胞迁移和凋亡;Western blot检测细胞中增殖细胞核抗原(PCNA)、基质金属蛋白酶-2(MMP-2)、MMP-9、半胱氨酸蛋白酶3(caspase-3)的表达;双荧光素酶报告基因实验验证LINC00342和miR-384的关系。结果: 与ctrl组、si-NC组比较,si-LINC00342组SK-MES-1细胞的OD450值、细胞侵袭数目、划痕愈合率、PCNA、MMP-2和MMP-9表达明显降低,细胞凋亡率、caspase-3表达明显上升(P<0.05);抑制miR-384表达减弱了敲低LINC00342对SK-MES-1细胞增殖、侵袭、迁移的抑制作用,降低了细胞凋亡能力;LINC00342靶向调控miR-384表达。结论: 敲低LINC00342可能通过靶向miR-384,进而抑制SK-MES-1细胞增殖、侵袭和迁移。

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	徐月亮
	王孝彬
	杨尧庆

关键词 ： LINC00342, miR-384, 肺鳞癌, 增殖, 侵袭, 迁移

Abstract：Objective: To investigate the influences of LINC00342 on the proliferation, invasion, and migration of lung squamous cell carcinoma through miR-384. Methods: Lung squamous cell carcinoma SK-MES-1 cells were grouped into the ctrl group (normally cultured SK-MES-1 cells), si-NC group (transfected with si-NC), and si-LINC00342 group (transfected with si-LINC00342), si-LINC00342+inhibitor-NC group (co-transfected with si-LINC00342 and inhibitor-NC), and si-LINC00342+ miR-384 inhibitor group (co-transfected with si-LINC00342 and miR-384 inhibitor); RT-qPCR was applied to detect the expression of LINC00342 and miR-384 in cells; CCK-8 assay and Transwell chamber were applied to detect cell proliferation and invasion; scratch assay and flow cytometry were used to cell migration and apoptosis; Western blot was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase-2 (MMP-2), MMP-9, and cysteine protease-3 (caspase-3) in cells; and dual-luciferase reporter assay was used to verify the relationship between LINC00342 and miR-384. Results: Compared with the ctrl group and si-NC group, the OD450 value, the number of cell invasions, the wound healing rate, the expressions of PCNA, MMP-2, and MMP-9 in SK-MES-1 cells in the si-LINC00342 group was obviously decreased, and the cell apoptosis was obviously decreased, the mortality rate and the expression of caspase-3 were obviously increased (P<0.05); inhibiting the expression of miR-384 attenuated the inhibitory effect of knockdown of LINC00342 on the proliferation, invasion, and migration of SK-MES-1 cells, and reduced the apoptosis ability; LINC00342 targeted miR-384 expression. Conclusion: Knockdown of LINC00342 may inhibit the proliferation, invasion, and migration of SK-MES-1 cells by targeting miR-384.

Key words： LINC00342 miR-384 Lung squamous cell carcinoma Proliferation Invasion Migration

基金资助:陕西省重点研发计划项目,(编号:2022SF-230)

通讯作者: 杨尧庆

引用本文:

徐月亮, 王孝彬, 杨尧庆. LINC00342调控miR-384对肺鳞癌细胞增殖侵袭迁移的影响研究[J]. 河北医学, 2023, 29(2): 177-182.
XU Yueliang, WANG Xiaobin, YANG Yaoqing. LINC00342 Regulates the Effect of miR-384 on the Proliferation Invasion and Migration of Lung Squamous Carcinoma Cells. HeBei Med, 2023, 29(2): 177-182.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2023.02.01 或 http://www.hbyxzzs.cn/CN/Y2023/V29/I2/177

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