Abstract：Objective: To explore the impacts and mechanism of long non-coding RNA (lncRNA LINC01194) on miR-302e in non-small cell lung cancer (NSCLC). Methods: Real-time fluorescence quantitative PCR (qRT-PCR) was applied to detect the expression of lncRNA LINC01194 and miR-302e in NSCLC tissue, paracancerous tissue, human lung normal epithelial cells BEAS-2B, and NSCLC cell lines, respectively, to screen the best intervention cell line; NCI-H1975 cells were divided into: control group (normal culture), si-NC group (transfected with si-NC), si-LINC01194 group (transfected with si-LINC01194), si-LINC01194+anti miR-NC group (co-transfected with si-LINC01194 and anti miR-NC), and si-LINC01194+anti miR-302e group (co-transfected with si-LINC01194 and anti miR-302e); qRT-PCR was applied to detect the expression of lncRNA LINC01194 and miR-302e in cells; MTT assay, plate cloning assay, Transwell chamber flow cytometry were applied to detect cell proliferation, migration, invasion and apoptosis, respectively; Western blot was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase-2 (MMP-2), B-cell lymphoma protein 2 (Bcl-2), and Bcl-2 associated X protein (Bax); and double Luciferase reporter gene experiment was applied to verify the targeting regulation relationship between lncRNA LINC01194 and miR-302e. Results: In NSCLC tissues and cells, the expression level of lncRNA LINC01194 increased, while the expression level of miR-302e decreased (P<0.05); inhibiting the expression of lncRNA LINC01194 was able to obviously inhibit the proliferation, migration, and invasion of NCI-H1975 cells, and promote cell apoptosis (P<0.05); inhibiting miR-302e expression was able to reverse the inhibitory effect of lncRNA LINC01194 expression on NCI-H1975 cell proliferation, migration, invasion, and promote cell apoptosis (P<0.05); double Luciferase reporter gene experiment confirmed that there was a targeted regulatory relationship between lncRNA LINC01194 and miR-302e. Conclusion: lncRNA LINC01194 is up-regulated and miR-302e is down-regulated in NSCLC tissues and cells, inhibiting lncRNA LINC01194 can up-regulate the expression of miR-302e, thereby inhibiting the proliferation, migration, and invasion of NSCLC cells and promoting their apoptosis.
马翾, 刘洋, 刘娟娟, 田浩, 李杰. LncRNA LINC01194在非小细胞肺癌中对miR-302e的影响及机制研究[J]. 河北医学, 2023, 29(10): 1600-1606.
MA Xuan, LIU Yang, LIU Juanjuan, et al. The Impacts and Mechanism of lncRNA LINC01194 on miR-302e in Non-Small Cell Lung Cancer. HeBei Med, 2023, 29(10): 1600-1606.
[1] Chen P,Liu Y,Wen Y,et al.Non-small cell lung cancer in China[J].Cancer Commun (Lond),2022,42(10):937-970. [2] Mithoowani H,Febbraro M.Non-small-cell lung cancer in 2022:a review for general practitioners in oncology[J].Curr Oncol,2022,29(3):1828-1839. [3] Wang M,Herbst R S,Boshoff C.Toward personalized treatment approaches for non-small-cell lung cancer[J].Nat Med,2021,27(8):1345-1356. [4] Saw S P L,Ong B H,Chua K L M,et al.Revisiting neoadjuvant therapy in non-small-cell lung cancer[J].Lancet Oncol,2021,22(11):501-516. [5] Liu Y,Liu J,Cui J,et al.Role of lncRNA LINC01194 in hepatocellular carcinoma via the miR-655-3p/SMAD family member 5 axis[J].Bioengineered,2022,13(1):1115-1125. [6] Song H R,Guo X B,Duan Y,et al.PAX5-induced upregulation of LINC01194 exerts oncogenic properties by regulating GOLPH3 expression via miR-486-5p in prostate cancer[J].Eur Rev Med Pharmacol Sci,2021,25(6):2528-2541. [7] Xing Z,Zhang Z,Gao Y,et al.The incRNA LINC01194/miR-486-5p axis facilitates malignancy in non-small cell lung cancer via regulating CDK4[J].Onco Targets Ther,2020,13(1):3151-3163. [8] Cui D,Qian R,Li Y.Circular RNA circ-CMPK1 contributes to cell proliferation of non-small cell lung cancer by elevating cyclin D1 via sponging miR-302e[J].Mol Genet Genomic Med,2020,8(2):999-1008. [9] Wang X,Liu Z,Tong H,et al.Linc01194 acts as an oncogene in colorectal carcinoma and is associated with poor survival outcome[J].Cancer Manag Res,2019,11(1):2349-2362. [10] Meng F,Zhou Y,Dong B,et al.Long non-coding RNA LINC01194 promotes the proliferation,migration and invasion of lung adenocarcinoma cells by targeting miR-641/SETD7 axis[J].Cancer Cell Int,2020,20(1):588-600. [11] Chen B,Song L,Nie X,et al.CXCL1 regulated by miR-302e is involved in cell viability and motility of colorectal cancer via inhibiting JAK-STAT signaling pathway[J].Front Oncol,2021,10(1):1-12 [12] Zou G,Zhang X,Wang L,et al.Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription[J].Theranostics,2020,10(15):6839-6853. [13] Li D,Jiang X,Zhang X,et al.Long noncoding RNA FGD5-AS1 promotes colorectal cancer cell proliferation,migration and invasion through upregulating CDCA7 via sponging miR-302e[J].2019,55(8):577-585.
2023, Vol. 29 
冀公网安备13080202000677号