Abstract：Objective: To study the effect of microRNA-122-5p on the expression, invasion and migration of MMP-9 in human colorectal cancer SW480 cells induced by TPA. Methods: Bioinformatics was used to screen colorectal cancer differential genes and predict their target miRNA. SW480 cells were treated with TPA (100 nM), and gene and protein expression levels were detected by RT-qPCR and Western blot; gelatin zymography was used to detect MMP-9 cell exocytosis; Transwell was used to detect cell invasion and migration ability. Results: MMP-9 was highly expressed in colon cancer tissues, and the upstream target gene miR-122-5P was screened; TPA induced a time-dependent upregulation of MMP-9 mRNA expression (P<0.01) and a downregulation of miR-122-5p expression (P<0.01); overexpression of miR-122-5p significantly downregulated TPA-induced MMP-9 mRNA and protein expression (P<0.05), the extracellular secretion of MMP-9 was reduced, and the invasion and migration ability of cells were significantly decreased (P<0.01). Conclusion: miR-122-5p may induce MMP-9 expression by regulating TPA and thus inhibit the invasive and migratory ability of colorectal cancer SW480 cells, providing a new idea to overcome the metastasis study of colorectal cancer.
[1] Wang MY,Qiu YH,Cai ML,et al.Role and molecular mechanism of stem cells in colorectal cancer initiation.[J].Drug Target,2020,(28):1-10. [2] Han K,Wang FW,Cao CH,et al.CircLONP2 enhances colorectal carcinoma invasion and metastasis through modulating the maturation and exosomal dissemination of microRNA-17[J].Mol Cancer,2020,(19):60. [3] Lu P,Chen J,Yan L,et al.RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-κB pathway[J].Cancer Biol Ther,2019,(20):435-443. [4] Kamiya T,Tanaka M,Hara H,et al.Inhibitory effects of 4-hydroperoxy-2-decenoic acid ethyl ester on phorbol ester- and TGF-β1-induced MMPs expression[J].Free Radic Res,2019,(53):1051-1059. [5] Lee CY,Chou YE,Hsin MC,et al.Dioscorea nipponica Makino suppresses TPA-induced migration and invasion through inhibition of matrix metalloproteinase-9 in human cervical cancer cells[J].Environ Toxicol,2020,(35):1194-1201. [6] Chen T,Lei S,Zeng Z,et al.MicroRNA 137 suppresses the proliferation,migration and invasion of cholangiocarcinoma cells by targeting WNT2B[J].Int Mol Med,2020,(45):886-896. [7] Ichikawa R,Kawasaki R,Iwata A,et al.MicroRNA 126 3p suppresses HeLa cell proliferation,migration and invasion,and increases apoptosis via the PI3K/PDK1/AKT pathway[J].Oncol Rep,2020,(43):1300-1308. [8] Xu Z,Liu G,Zhang M,et al.miR-122-5p inhibits the proliferation,invasion and growth of bile duct carcinoma cells by targeting ALDOA[J].Cell Physiol Biochem,2018,(48):2596-2606. [9] Dong H,Diao H,Zhao Y,et al.Overexpression of matrix metalloproteinase-9 in breast cancer cell lines remarkably increases the cell malignancy largely via activation of transforming growth factor beta/SMAD signalling[J].Cell Prolif,2019,(52):12633. [10] Wu Y,Pu N,Su W,et al.Downregulation of miR-1 in colorectal cancer promotes radioresistance and aggressive phenotypes[J].Cancer,2020,(11):4832-4840. [11] Yoon JH,Pham TH,Lee J,et al.Methyl linderone suppresses TPA-stimulated IL-8 and MMP-9 expression via the ERK/STAT3 pathway in MCF-7 breast cancer cells[J].Microbiol Biotechnol,2020,(30):325-332. [12] Tsai JY,Rédei D,Hohmann J,et al.12-Deoxyphorbol esters induce growth arrest and apoptosis in human lung cancer A549 cells via activation of PKC-δ/PKD/ERK signaling pathway[J].Int Mol Sci,2020,(21). [13] Jiang S,Li Q,Liu Y,Zhang H,et al.Activation of WNT7b autocrine eases metastasis of colorectal cancer via epithelial to mesenchymal transition and predicts poor prognosis[J].BMC Cancer,2021,(21):180. [14] Tuo L,Chu X,Sha S,et al.MicroRNA and lung cancer:a mini review[J].Zhongguo Fei Ai Za Zhi,2018,(21):727-730.
2023, Vol. 29 
冀公网安备13080202000677号