lncRNA MNX1-AS1在非小细胞肺癌中的表达及调控PI3K/AKT通路对癌细胞生物学行为的影响

doi:10.3969/j.issn.1006-6233.2023.01.003

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摘要 目的: 探讨长链非编码RNA(lncRNA)MNX1-AS1(lncRNA MNX1-AS1)在非小细胞肺癌(NSCLC)中的生物学功能及可能的作用机制。方法: 选取2017年6月至2019年3月期间在本院进行手术治疗的75例NSCLC患者癌组织及癌旁组织标本,RT-qPCR法检测标本lncRNA MNX1-AS1表达水平,分析lncRNA MNX1-AS1表达与NSCLC临床病理特征及预后的相关性;体外培养NSCLC细胞系PC-9细胞,分别转染si-MNX1-AS1(si-MNX1-AS1组)、si-NC(si-NC组),MTT法及EdU实验检测各组PC-9细胞增殖;Transwell小室法检测细胞迁移与侵袭;Western blot实验检测PI3K/AKT通路相关蛋白表达。结果: lncRNA MNX1-AS1在NSCLC癌组织与细胞系中表达水平显著升高(P<0.05)。lncRNA MNX1-AS1表达与TNM分期和淋巴转移有关(P<0.05),Kaplan-Meier生存分析显示,lncRNA MNX1-AS1高表达组患者3年累积生存率显著低于低表达组,差异有统计学意义(42.11% vs 81.08%,χ²=11.132,P=0.001);与si-NC组比较,si-MNX1-AS1组PC-9细胞的增殖、迁移与侵袭能力以及p-PI3K、p-AKT、p-mTOR蛋白表达水平显著降低(P<0.05)。结论: lncRNA MNX1-AS1在NSCLC中表达水平升高,可能通过激活PI3K/AKT通路影响NSCLC细胞的增殖、迁移、侵袭,促进NSCLC的发生发展。

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	刘桂霞
	邓科兰
	厉银平

关键词 ：非小细胞肺癌, 长链非编码RNA MNX1-AS1, PI3K/AKT通路, 迁移, 侵袭

Abstract：Objective: To investigate the biological function and possible mechanism of long non-coding RNA (lncRNA) MNX1-AS1 (lncRNA MNX1-AS1) in non-small cell lung cancer (NSCLC). Methods: The cancer tissue and adjacent tissue samples of 75 NSCLC patients who underwent surgical treatment in our hospital from June 2017 to March 2019 were taken. RT-qPCR method was performed to detect the expression level of lncRNA MNX1-AS1 in the samples, the correlation between the expression of lncRNA MNX1-AS1 and the clinicopathological characteristics and prognosis of NSCLC was analyzed; the NSCLC cell line PC-9 cells were cultured in vitro and transfected with si-MNX1-AS1 (si-MNX1-AS1 group) and si-NC (si-NC group), respectively. the proliferation of PC-9 cells in each group was detected by MTT assay and EdU assay; Transwell chamber assay was performed to detect cell migration and invasion; Western blot assay was performed to detect the expression of PI3K/AKT pathway-related proteins. Results: The expression level of lncRNA MNX1-AS1 was obviously higher in NSCLC cancer tissues and cell lines (P<0.05). The expression of lncRNA MNX1-AS1 was associated with TNM stage and lymphatic metastasis (P<0.05), Kaplan-Meier survival analysis showed that the 3-year cumulative survival rate of patients in the high expression group of lncRNA MNX1-AS1 was obviously lower than that in the low expression group (42.11% vs 81.08%, χ²=11.132, P=0.001); compared with the si-NC group, the proliferation, migration and invasion abilities of PC-9 cells in the si-MNX1-AS1 group and the protein expression levels of p-PI3K, p-AKT and p-mTOR were obviously decreased (P<0.05). Conclusion: The expression level of lncRNA MNX1-AS1 is increased in NSCLC, which may affect the proliferation, migration and invasion of NSCLC cells by activating the PI3K/AKT pathway, and promote the occurrence and development of NSCLC.

Key words： Non-small cell lung cancer Long non-coding RNA MNX1-AS1 PI3K/AKT pathway Migration Invasion

基金资助:2020年度孝感市自然科学计划项目,(编号:XGKJ2020010008)

通讯作者: 厉银平

引用本文:

刘桂霞, 邓科兰, 厉银平. lncRNA MNX1-AS1在非小细胞肺癌中的表达及调控PI3K/AKT通路对癌细胞生物学行为的影响[J]. 河北医学, 2023, 29(1): 13-18.
LIU Guixia, DENG Kelan, LI Yinping. Expression of lncRNA MNX1-AS1 in Non-Small Cell Lung Cancer and the Effect of Regulation of PI3K/AKT Pathway on the Biological Behavior of Cancer Cells. HeBei Med, 2023, 29(1): 13-18.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2023.01.003 或 http://www.hbyxzzs.cn/CN/Y2023/V29/I1/13

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