circ_0011385和miR-330-3p在结肠癌中的表达及其对结肠癌细胞恶性行为的影响

doi:10.3969/j.issn.1006-6233.2022.06.07

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摘要 目的: 探讨结肠癌组织中circ_0011385和miR-330-3p表达情况,研究circ_0011385靶向miR-330-3p对结肠癌细胞(SW1116)恶性行为的影响。方法: RT-qPCR检测circ_0011385和miR-330-3p在结肠癌组织中表达情况,并分析circ_0011385和miR-330-3p表达水平与结肠癌患者临床病理特征的关系。皮尔森相关分析确定circ_0011385和miR-330-3p表达的相关性。circ_0011385和miR-330-3p的关系通过双荧光素酶报告实验来确定。将si-circ_0011385、si-NC、si-circ_0011385+anti-miR-NC、si-circ_0011385+anti-miR-330-3p分别转染SW1116细胞,Transwell、流式细胞术分析细胞迁移、侵袭和凋亡。结果: 结肠癌组织中circ_0011385呈高表达,miR-330-3p呈低表达,二者的表达水平呈负相关关系(r=-0.8924)。circ_0011385和miR-330-3p表达水平与结肠癌患者年龄、肿瘤大小无相关性(P>0.05),与分化程度、TNM分期、淋巴结转移显著相关(P<0.05)。miR-330-3p是circ_0011385的靶基因。沉默circ_0011385表达后SW1116细胞凋亡率、miR-330-3p表达水平显著升高(P<0.05),细胞迁移数、侵袭数显著减少(P<0.05)。抑制miR-330-3p表达显著减弱沉默circ_0011385对SW1116细胞凋亡、迁移和侵袭的作用(P<0.05)。结论: 沉默circ_0011385通过上调miR-330-3p表达来诱导结肠癌细胞凋亡并抑制其迁移和侵袭。

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	东金花
	吴葆华
	闫丹措

关键词 ：结肠癌, circ_0011385, miR-330-3p, 凋亡, 迁移, 侵袭

Abstract：Objective: To investigate the expression of circ_0011385 and miR-330-3p in colon cancer tissues, and further study the effect of circ_0011385 targeting miR-330-3p on the malignant behavior of colon cancer cells (SW1116). Methods: RT-qPCR was performed to detect circ_0011385 and miR-330-3p expression in colon cancer tissues, and further analyze the relationship between the expression levels of circ_0011385 and miR-330-3p and the clinico- pathological characteristics of colon cancer patients. The relationship between circ_0011385 and miR-330-3p was confirmed by dual luciferase reporter assays. SW1116 cells were transfected with si-circ_0011385, si-NC, si-circ_0011385+anti-miR-NC, or si-circ_0011385+anti-miR-330-3p, respectively. Transwell and flow cytometry analysis were used to analyze cell migration, invasion and apoptosis. Results: In colon cancer tissues, circ_0011385 was highly expressed, while miR-330-3p was lowly expressed, and the expression levels of the two were negatively correlated (r=-0.8924). Circ_0011385 and miR-330-3p expression was not correlated with the age and tumor size of colon cancer patients (P>0.05), but it was significantly correlated with the degree of differentiation, TNM stage, and lymph node metastasis (P<0.05). MiR-330-3p is a target gene of circ_0011385. After silencing the expression of circ_0011385, the apoptosis rate of SW1116 cells and the expression level of miR-330-3p were significantly increased (P<0.05), and the number of cell migration and invasion was significantly reduced (P<0.05). Inhibition of miR-330-3p expression significantly reduced the effect of silencing circ_0011385 on SW1116 cell apoptosis, migration and invasion (P<0.05). Conclusion: Circ_0011385 is highly expressed, while miR-330-3p is lowly expressed in colon cancer. Silencing circ_0011385 induces cell apoptosis and inhibit the migration and invasion in colon cancer by up-regulating miR-330-3p expression.

Key words： Colon cancer Circ_0011385 MiR-330-3p Apoptosis Migration Invasion

基金资助:青海省重点研发与转化计划项目,(编号:175110013106445)

通讯作者: 闫丹措

引用本文:

东金花, 吴葆华, 闫丹措. circ_0011385和miR-330-3p在结肠癌中的表达及其对结肠癌细胞恶性行为的影响[J]. 河北医学, 2022, 28(6): 909-913.
DONG Jinhua, WU Baohua, YAN dancuo. Expression of Circ_0011385 and MiR-330-3p in Colon Cancer and the Influence on the Malignant Behavior of Colon Cancer Cells. HeBei Med, 2022, 28(6): 909-913.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.06.07 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I6/909

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