Abstract：Objective: To investigate the effect of Allicin on endometriosis (EMs) in rats and its mechanism. Methods: The EMs rat model was prepared by auto-internal membrane transplantation, and the 30 model rats were randomly divided into model group, Allicin (10mg/kg) group and Allicin (10mg/kg) + LY294002 (PI3K specific inhibitor, 40mg/kg) group, n=10; another 10 rats were set as the sham operation group. The drugs were given once daily by intraperitoneal injection in each group (the rats in sham operation group and model group were given normal saline) for 4 weeks. The adhesion scores of endometriosis lesions was detected, the lesion volume was measured by calipers, the endometrium pathological examination was examed by HE staining method, the content of TNF-α, IL-1β, IL-6, IFN-γ were detected by ELISA method, the content of MDA and the activity of MPO, SOD, CAT in endometriosis lesions were detected by biochemical analysis method; the expression of Akt, p-Akt, Nrf2, HO-1, NF-κB were detected by Western blot method.Results: Compared with the sham operation group, the interstitium of endometriosis lesions in the model group is dense, blood vessels are proliferated and inflammatory cell infiltration changes. The contents of TNF-α, IL-1β, IL-6, IFN-γ, MDA in the ectopic lesion tissue were increased and the activities of MPO, SOD, CAT were decreased (P<0.01); the expression of p-Akt, Nrf2, HO-1 were down-regulated and the ratio of p-Akt/Akt was decreased (P<0.01), while the expression of NF-κB was up-regulated (P<0.01). Compared with the model group, the lesion volume and adhesion score were significantly decreased in Allicin group and Allicin + LY294002 group (P<0.05 or P<0.01); ectopic lesions include epithelial cell shedding, interstitial porosity, neovascularization; the content of TNF-α, IL-1β, IL-6, IFN-γ, MDA were decreased while the activities of MPO, SOD, CAT were increased (P<0.05 or P<0.01); the expression of p-Akt, Nrf2, HO-1 were up-regulated and the ratio of p-Akt/Akt was increased (P<0.01), while the expression of NF-κB was down-regulated (P<0.01). Compared with the Allicin group, the lesion volume and adhesion score were significantly increased in Allicin + LY294002 group (P<0.01), the angiogenesis in ectopic lesions was increased; the content of TNF-α, IL-1β, IL-6, IFN-γ, MDA were increased and the activities of MPO, SOD, CAT were decreased (P<0.01); the expression of p-Akt, Nrf2, HO-1, NF-κB were down-regulated and the ratio of p-Akt/Akt was decreased (P<0.01), while the expression of NF-κB was up-regulated (P<0.01).Conclusion: Allicin has a certain therapeutic effect on EMs in rats; which mechanism may be related to the activation of PI3K/Akt/Nrf2 pathway, thereby inhibiting inflammation and oxidative stress.
[1] 张真真,桂涛,汤伟伟,等.桂枝茯苓丸抑制子宫内膜异位症模型大鼠异位病灶侵袭转移的机制[J].中医学报,2020,35(11):2397~2401. [2] 龙宁,刘跃辉,徐凡,等.子宫内膜异位症患者凝血指标、炎性因子和氧化应激指标表达水平的价值[J].河北医药,2020,42(8):1152~1155. [3] 陈慧,郑晓梅,夏晓,等.PI3K/Akt信号通路对脐血间充质干细胞减轻缺血性脑损伤和炎症反应的调节作用[J].中国免疫学杂志,2021,37(2):155~160. [4] 杨庆辉,路平,姬颖华.大蒜素对前列腺癌患者细胞免疫功能的影响[J].世界中医药,2018,13(3):605~608. [5] 凌书建,杨丽萍,陶剑光,等.大蒜素对大鼠深Ⅱ度烧伤创面氧化应激和炎症反应的影响[J].中国现代中药,2021,23(1):77~82. [6] 孙瑞英,崔轶凡.子宫内膜异位症动物模型制备方法研究概况[J].山西中医药大学学报,2020,21(6):457~460,464. [7] Xu Y,Li L.Primary squamous cell carcinoma arising from endometriosis of the ovary:a case report and literature review [J].Curr Probl Cancer,2018,42(3):329~336. [8] Samimi M,Pourhanifeh MH,Mehdizadehkashi A,et al.The role of inflammation,oxidative stress,angiogenesis,and apoptosis in the pathophysiology of endometriosis:basic science and new insights based on gene expression [J].Cell Physiol,2019,234(11):19384~19392. [9] Amreen S,Kumar P,Gupta P,et al.Evaluation of oxidative stress and severity of endometriosis [J].Hum Reprod Sci,2019,12(1):40~46. [10] Deshmukh P, Unni S, Krishnappa G,et al.The Keap1-Nrf2 pathway:promising therapeutic target to counteract ROS-mediated damage in cancers and neuro degenerative diseases [J].Biophys Rev,2017,9(1):41~56.
2022, Vol. 28 
冀公网安备13080202000677号