Abstract：Objective: To investigate the effect and mechanism of carnosine on vascular calcification in β-glycerophosphate (β-GP)-induced vascular smooth muscle cells (VSMCs). Methods: Primary isolated and cultured rat aortic VSMCs were divided into 3 groups, the control group (normal culture medium), calcification group (10mM β-GP), and carnosine group (10mM β-GP+10mM carnosine). The cells were treated consecutively for 10 days. Alkaline phosphatase (ALP) activities and alizarin red staining were used to detect the calcification of VSMCs. The expression levels of BMP-2, Runx2, Cleaved-caspase3, β-catenin and GSK-3β(Ser9) proteins in the VSMCs in various groups were measured by Western blotting method. Results: After β-GP treatment, calcification occured in VSMCs. Compared with control group, calcium salt deposition and ALP activity increased significantly, and osteogenic marker BMP-2 and Runx2 proteins were significantly upregulated in calcification group. Compared with calcification group, carnosine markably reduced the calcium salt deposition, inactivated the ALP activity, and downregulated BMP-2 and Runx2 proteins. The apoptosis result showed that Cleaved-caspase3 protein was increased in calcification group and decreased in carnosine group. The signal pathway protein analysis revealed that β-GP downregulated GSK-3β(Ser9) and upregulated β-catenin, while carnosine reversed the proteins expression obviously. Conclusion: Carnosine inhibits VSMCs calcification induced by hyperphosphate and may be related to inhibition of β-catenin signaling pathway and its upregulating osteogenic factor transcription and apoptosis.
金朝霞, 徐成胜. 肌肽对血管平滑肌细胞钙化的影响及机制研究[J]. 河北医学, 2022, 28(3): 358-362.
JIN Zhaoxia, XU Chengsheng. Study of the Effect of Carnosine on Vascular Smooth Muscle Cell Calcification and Its Mechanism. HeBei Med, 2022, 28(3): 358-362.
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2022, Vol. 28 
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