Abstract：Objective: To investigate the effect of microRNA-182 (miR-182) on the proliferation, apoptosis and migration of prostate cancer PC-3 cells and its possible mechanism. Methods: Prostate cancer PC-3 cells in logarithmic growth stage were randomly divided into control group, miR-182 low expression vector (miR-182 inhibitor) group and miR-182 overexpression vector (miR-182 mimics) group. After corresponding treatment, each group was cultured for 72 hours; the cell survival rate, monoclonal forming number, apoptosis rate, migration ability, miR-182, metadherin (MTDH) mRNA and protein expression level were measured. Results: Compared with the control group, the cell survival rate, monoclonal forming number, number of membrane penetration, the expression levels of miR-182, MTDH mRNA and protein in miR-182 inhibitor group were significantly lower (P<0.05), and the apoptosis rate was significantly higher (P<0.05); the cell survival rate, monoclonal forming number, number of membrane penetration, the expression levels of miR-182, MTDH mRNA and protein in miR-182 mimics group were significantly higher (P<0.05), and the apoptosis rate was significantly lower (P<0.05). Compared with miR-182 inhibitor group, the cell survival rate, monoclonal forming number, number of membrane penetration, the expression levels of miR-182, MTDH mRNA and protein in miR-182 mimics group were significantly higher (P<0.05), and the apoptosis rate was significantly lower (P<0.05). Conclusion: Down regulation of miR-182 expression can inhibit the proliferation, migration and induce apoptosis of prostate cancer PC-3 cells; the mechanism may be related to the inhibition of MTDH mRNA and protein expression; up regulation of miR-182 expression can reverse the biological behavior of prostate cancer PC-3 cells.
甘卓, 李艳平, 史冰, 王群. miR-182对前列腺癌PC-3细胞增殖凋亡迁移的影响及机制研究[J]. 河北医学, 2022, 28(3): 353-357.
GAN Zhuo, LI Yanping, SHI Bing, et al. Effect of MiR-182 on the Proliferation Apoptosis and Migration of Prostate Cancer PC-3 Cells and Its Mechanism. HeBei Med, 2022, 28(3): 353-357.
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2022, Vol. 28 
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