2025年4月4日 星期五
首页        期刊介绍        编委会        投稿指南        期刊订阅        广告合作        联系我们        English
河北医学  2022, Vol. 28 Issue (3): 353-357    DOI: 10.3969/j.issn.1006-6233.2022.03.01
  论著 本期目录 | 过刊浏览 | 高级检索 |
miR-182对前列腺癌PC-3细胞增殖凋亡迁移的影响及机制研究
甘卓, 李艳平, 史冰, 王群
湖南中医药大学第一附属医院核医学科, 湖南 长沙 410007
Effect of MiR-182 on the Proliferation Apoptosis and Migration of Prostate Cancer PC-3 Cells and Its Mechanism
GAN Zhuo, LI Yanping, SHI Bing, et al
The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Hunan Changsha 410007, China
全文: PDF (1661 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 目的:探讨微小RNA-182(miR-182)对前列腺癌PC-3细胞增殖、凋亡、迁移的影响及可能的作用机制。方法:将对数生长期的前列腺癌PC-3细胞随机分为:对照组、miR-182低表达载体(miR-182 inhibitor)组、miR-182过表达载体(miR-182 mimics)组,各组进行相应处理后,培养72h;测定细胞存活率、单克隆形成数目、凋亡率、迁移能力、miR-182、异黏蛋白(MTDH)mRNA和蛋白表达水平。结果:与对照组比较,miR-182 inhibitor组细胞存活率、单克隆形成数目、穿膜数、miR-182、MTDH mRNA和蛋白表达水平明显降低(P<0.05),细胞凋亡率明显升高(P<0.05);miR-182 mimics组细胞存活率、单克隆形成数目、穿膜数、miR-182、MTDH mRNA和蛋白表达水平明显升高(P<0.05),细胞凋亡率明显降低(P<0.05)。与miR-182 inhibitor组比较,miR-182 mimics组细胞存活率、单克隆形成数目、穿膜数、miR-182、MTDH mRNA和蛋白表达水平明显升高(P<0.05),细胞凋亡率明显降低(P<0.05)。结论:下调miR-182表达可抑制前列腺癌PC-3细胞增殖、迁移,诱导其凋亡;其机制可能与抑制MTDH mRNA和蛋白的表达有关;上调miR-182表达可逆转前列腺癌PC-3细胞的生物学行为。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
甘卓
李艳平
史冰
王群
关键词 微小RNA-182前列腺癌PC-3细胞增殖凋亡迁移异黏蛋白    
AbstractObjective: To investigate the effect of microRNA-182 (miR-182) on the proliferation, apoptosis and migration of prostate cancer PC-3 cells and its possible mechanism. Methods: Prostate cancer PC-3 cells in logarithmic growth stage were randomly divided into control group, miR-182 low expression vector (miR-182 inhibitor) group and miR-182 overexpression vector (miR-182 mimics) group. After corresponding treatment, each group was cultured for 72 hours; the cell survival rate, monoclonal forming number, apoptosis rate, migration ability, miR-182, metadherin (MTDH) mRNA and protein expression level were measured. Results: Compared with the control group, the cell survival rate, monoclonal forming number, number of membrane penetration, the expression levels of miR-182, MTDH mRNA and protein in miR-182 inhibitor group were significantly lower (P<0.05), and the apoptosis rate was significantly higher (P<0.05); the cell survival rate, monoclonal forming number, number of membrane penetration, the expression levels of miR-182, MTDH mRNA and protein in miR-182 mimics group were significantly higher (P<0.05), and the apoptosis rate was significantly lower (P<0.05). Compared with miR-182 inhibitor group, the cell survival rate, monoclonal forming number, number of membrane penetration, the expression levels of miR-182, MTDH mRNA and protein in miR-182 mimics group were significantly higher (P<0.05), and the apoptosis rate was significantly lower (P<0.05). Conclusion: Down regulation of miR-182 expression can inhibit the proliferation, migration and induce apoptosis of prostate cancer PC-3 cells; the mechanism may be related to the inhibition of MTDH mRNA and protein expression; up regulation of miR-182 expression can reverse the biological behavior of prostate cancer PC-3 cells.
Key wordsMicroRNA-182    Prostate cancer PC-3 cells    Proliferation    Apoptosis    Migration    Metadherin
    
基金资助:湖南省卫生健康委科研立项课题,(编号:20200469)
通讯作者: 李艳平   
引用本文:   
甘卓, 李艳平, 史冰, 王群. miR-182对前列腺癌PC-3细胞增殖凋亡迁移的影响及机制研究[J]. 河北医学, 2022, 28(3): 353-357.
GAN Zhuo, LI Yanping, SHI Bing, et al. Effect of MiR-182 on the Proliferation Apoptosis and Migration of Prostate Cancer PC-3 Cells and Its Mechanism. HeBei Med, 2022, 28(3): 353-357.
链接本文:  
http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.03.01     或     http://www.hbyxzzs.cn/CN/Y2022/V28/I3/353
冀ICP备2025106803号    冀公网安备13080202000786号
版权所有 © 2016 《河北医学》杂志社
本系统由北京玛格泰克科技发展有限公司设计开发