Abstract：Objective: To study the changes of serum CXCL chemokine ligand-10 (CXCL-10) and interleukin-27 (IL-27) levels in diarrhea patients with Clostridium difficile infection (CDI) and their correlation with the severity of the disease. Metods: A total of 150 patients with diarrhea were divided into observation group (positive Cd culture,n=90) and control group (negative Cd culture,n=60) according to the results of Cd culture. The patients in observation group were further divided into mild subgroup (score<4,n=38) and moderate-severe subgroup (score≥4,n=52) according to the severity score (SSI) of CDI. The serum levels of CXCL-10 and IL-27 were detected in all the three groups, and the correlation between the serum levels of CXCL-10 and IL-27 and the severity of CDI diarrhea was analyzed. Results: The serum levels of CXCL-10 and IL-27 in the three groups were as follows: observation group>control group>healthy group (P<0.05);the serum levels of CXCL-10 and IL-27 in the moderate-severe subgroup were significantly higher than those in the mild subgroup (P<0.05).The SSI score of the observation group was (4.68±1.73), the SSI score of the mild subgroup was (3.02±0.89), and the SSI score of the moderate-severe subgroup was (5.13±1.09). Spearman linear correlation analysis showed that there was a positive correlation between serum CXCL-10 and IL-27 levels and SSI score in the observation group (r=0.317 and 0.368,P=0.002 and 0.000). ROC curve analysis showed that the area under curve (AUC) of serum CXCL-10, IL-27 and their combination in the diagnosis of CDI diarrhea were 0.846, 0.853 and 0.874 respectively, the diagnostic sensitivity was 85.25%, 80.30% and 82.75% respectively, and the diagnostic specificity was 78.50%, 82.55% and 85.85% respectively. Conclusion: Serum CXCL-10 and IL-27 can help judge the severity of diarrhea in patients with Clostridium difficile infection, which has clinical application value.
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2022, Vol. 28 
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