基于PPAR-α/PGC-1α途径探讨低氧减重对肥胖大鼠能量代谢的影响

doi:10.3969/j.issn.1006-6233.2022.12.007

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摘要 目的: 探讨低氧运动对肥胖大鼠能量代谢的影响及其作用机制。方法: 80只雄性SD大鼠随机分为普通膳食对照组(10只)、高脂膳食模型组(70),模型组大鼠给予7周高脂膳食诱导,构建肥胖大鼠模型,取建模成功的肥胖大鼠60只,随机分为常氧安静组(A组)、常氧运动组(AE组)、16.3%低氧安静组(B组)、16.3%低氧运动组(BE组)、13.3%低氧安静组(C组)、13.3%低氧运动组(CE组),每组10只,继续进行高脂膳食饲养,运动组大鼠进行跑台耐力训练,测量大鼠体长、体重,计算Lee's指数;检测血糖(BG)和血脂四项(BG、TC、LDL-C、TG);采用大鼠代谢系统检测运动后耗氧量、能量消耗;检测骨骼肌组织中PPAR-α、PGC-1α蛋白表达。结果: 7周高脂膳食饲养后,大鼠体重明显增加,且超过对照组大鼠体重的20%,血糖、TC、TG、LDL-C含量均明显增加(P<0.01,P<0.05),提示营养性肥胖大鼠模型建立成功。采用低氧、耐力运动干预后8周后,与A组比较,AE、BE、C、CE组大鼠的体重增长、TC、TG、LDL-C、血糖均明显下降(P<0.05,P<0.01),BE、CE组大鼠HDL-C明显升高(P<0.01);与AE组比较,BE、CE组大鼠体重增长、TC、TG、LDL-C、血糖明显降低(P<0.01),BE、CE组大鼠HDL-C明显升高(P<0.01);与A组相比,AE、B、BE、C、CE组大鼠运动即刻耗氧量、耗能量呈现增加趋势(P<0.01,P<0.05);与AE组相比,BE、CE组大鼠运动即刻耗氧量、耗能量呈现增加趋势(P<0.01或P<0.05);与A组相比,BE、C、CE组大鼠PPAR-α、PGC-1α蛋白表达明显升高(P<0.05,P<0.01);与AE组相比,BE、C、CE大鼠PPAR-α、PGC-1α蛋白表达明显增加(P<0.05,P<0.01)。结论: 低氧运动可能增强骨骼肌PGC-1α、PPAR-α蛋白的表达,进而改善肥胖大鼠的能量代谢水平。

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关键词 ：低氧耐力运动, 营养性肥胖, 能量代谢, PPAR-α, PGC-1α

Abstract：Objective: To study the mechanism of hypoxic exercise energy metabolism in obese rats . Methods: Eighty male SD rats were randomly divided into normal diet control group (10 rats) and high-fat diet model group (70 rats), and the model group rats were induced with high-fat diet for 7 weeks to construct an obese rat model. 60 obese rats with successful modeling were randomly divided into normoxic quiet group (group A), normoxic exercise group (group AE), 16. 3% hypoxic quiet group (group B), 16. 3% hypoxic exercise group (group BE), 13. 3% hypoxic quiet group (group C), and 13. 3% hypoxic exercise group (group CE). group), 13. 3% hypoxic quiet group (group C), 13. 3% hypoxic exercise group (group CE), 10 rats in each group, continued to be fed with high-fat diet, and the rats in the exercise group were trained with running platform endurance, and body length and body weight were measured, and Lee's index was calculated. Blood glucose (BG) and lipid quadruple (BG, TC, LDL-C, TG) were detected; oxygen consumption and energy expenditure after exercise were detected using rat metabolic system; PPAR-α and PGC-1α protein expression in skeletal muscle tissue were detected. Results: After 7 weeks of high-fat dietary feeding, the rats' body weight increased significantly and exceeded 20% of the body weight of the control rats, and blood glucose, TC, TG, and LDL-C contents increased significantly (P<0. 01, P<0. 05), suggesting that the nutritional obesity rat model was successfully established. After 8 weeks of intervention with hypoxia and endurance exercise, the body weight gain, TC, TG, LDL-C, and blood glucose of rats in AE, BE, C, and CE groups were significantly decreased (P<0. 05, P<0. 01), and HDL-C of rats in BE and CE groups were significantly increased (P<0. 01) compared with group A. Compared with group AE, the body weight gain, TC, TG, LDL-C and blood glucose of rats in groups BE and CE were significantly lower (P<0. 01), and the HDL-C of rats in groups BE and CE was significantly higher (P<0. 01); compared with group A, the immediate oxygen consumption and energy consumption of rats in the AE, B, BE, C and CE groups showed an increasing trend (P<0. 01, P<0. 05); compared with group AE, groups BE and CE showed an increase in oxygen consumption and energy consumption immediately after exercise (P<0. 05); compared with group A, the expression of PPAR-α and PGC-1α protein in groups BE, C and CE was significantly increased (P<0. 05, P<0. 01); compared with group AE, the expression of PPAR-α and PGC-1α protein in the BE, C and CE rats was significantly increased (P<0. 05, P<0. 01). Protein expression was significantly increased in BE, C and CE rats compared with group AE (P<0. 05, P<0. 01). Conclusion: Hypoxic exercise may enhance the expression of PGC-1α and PPAR-α proteins in skeletal muscle and thus improve the energy metabolism level in obese rats.

Key words： Hypoxia exercise Nutritional obesity Energy metabolism PPAR-a PGC-1α

基金资助:青海省科技项目,(编号:2016-ZJ-707)

通讯作者: 杨历新

引用本文:

郜琨, 杨历新, 王叶, 李正, 李小鸾. 基于PPAR-α/PGC-1α途径探讨低氧减重对肥胖大鼠能量代谢的影响[J]. 河北医学, 2022, 28(12): 1978-1984.
GAO Kun, YANG Lixin, WANG Ye, et al. The Effect of Hypoxia Exercise on Energy Metabolism in Obese Rats Based on PPAR-α/PGC-1α Pathway. HeBei Med, 2022, 28(12): 1978-1984.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2022.12.007 或 http://www.hbyxzzs.cn/CN/Y2022/V28/I12/1978

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