Abstract：Objective: To observe the expression of miR-762 in plasma of patients with acute lung injury and explore its relationship with potential value.Methods: A total of 60 patients with lung injury admitted to our hospital from September 2018 to September 2021 were collected and divided into medium low risk group and high risk group according to clinical symptoms and mMRC scores. Scores were analyzed with acute physiology and chronic health evaluation (APACHE Ⅱ). Human soluble myeloid cell trigger receptor-1 (sTREM-1) kit and human soluble FMS like tyrosine kinase 1 (sFlt-1) enzyme linked immunosorbent assay (ELISA) kit were used to detect the contents of sTREM-1, sFlt-1 in plasma; real time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of miR-762 in plasma. Receiver operating characteristic curve (ROC) was plotted to judge the diagnostic value and cut-off point of miR-762 in patients with lung injury. Results: The contents of sTREM-1 and sFlt-1, the relative expression of miR-762 of high-risk group and middle-low-risk group were all significantly higher than those of normal group. Compared with the middle-low-risk group, the contents of sTREM-1, sFlt-1 and the relative expression of miR-762 were substantially increased in the high-risk group in plasma. ROC curve showed that the relative expression of miR-762 was used to predict the area under curve (AUC) = 0.987, 95% confidence interval (CI): (0.9688 - 1.000), and the cut-off point was 1.210; AUC = 1.00, 95% CI: (1.000-1.000), with a cut-off point of 2.396. The scores of miR-762 and APACHE Ⅱ markedly increased, and the contents of sTREM-1 and sFlt-1 were positively correlated. The correlation coefficients (r) were 0.386, 0.321, 0.432. Conclusion: The high expression of miR-762 in plasma from patients with acute lung injury was related to the aggravation of lung injury, and it was a predictive marker of acute lung injury.
2022, Vol. 28 
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