Abstract：Objective: To explore the effect of circ_0000615 on the proliferation, migration and invasion of osteosarcoma cells and its molecular mechanism.Methods: The osteosarcoma tissues and adjacent tissues of 60 patients with osteosarcoma were selected, and real-time fluorescent quantitative PCR (RT-qPCR) was used to detect the expression levels of circ_0000615 and miR-142-3p; the osteosarcoma cells U2OS were divided into si-circ_0000615 group, si-NC group, miR-142-3p group, miR-NC group, si-circ_0000615+anti-miR-142-3p group, si-circ_0000615+anti-miR-NC group; tetramethylazolium salt colorimetric method (MTT) to detect cell inhibition rate; clone formation experiment to detect cell clone number; Transwell method to detect U2OS cell migration and invasion numbers; Western blot method to detect protein expression; dual luciferase reporter experiment to detect the targeting relationship between circ_0000615 and miR-142-3p. Results: Compared with the adjacent tissues, the expression level of circ_0000615 in osteosarcoma tissue was increased, and the expression level of miR-142-3p was decreased (P<0.05). After silencing circ_0000615 or overexpressing miR-142-3p, the inhibition rate of U2OS cells was increased, the number of clones, migration and invasion of U2OS cells were decreased, the expression level of E-cadherin was increased, and the expression level of N-cadherin was decreased (P<0.05). circ_0000615 targets and regulates miR-142-3p; inhibition of miR-142-3p can reduce the inhibitory effect of circ_0000615 on U2OS cell proliferation, migration and invasion. Conclusion: Silencing circ_0000615 can inhibit the proliferation, migration and invasion of osteosarcoma cells through targeted regulation of miR-142-3p.
李贺伟, 阮锋. circ_0000615靶向调控miR-142-3p对骨肉瘤细胞增殖迁移侵袭的影响[J]. 河北医学, 2022, 28(11): 1774-1777.
LI Hewei, RUAN Feng. Effect of circ_0000615 on Proliferation Migration and Invasion of Osteosarcoma Cells by Targeting miR-142-3p. HeBei Med, 2022, 28(11): 1774-1777.
[1] 李胜,王威,李建军.骨肉瘤靶向治疗研究进展[J].山东医药,2019,59(22):99-102. [2] 侯静雨,王楚楚,姚伟涛.骨肉瘤的基因组学及靶向治疗研究进展[J].肿瘤研究与临床,2020,32(9):658-662. [3] Zhang G,Li Y,Xu J,et al.Advances in the role of miRNAs in the occurrence and development of osteosarcoma[J].Open Med,2020,15(1):1003-1011. [4] Xu GX,Wang J,Jia YX,et al.miR-142-3p functions as a potential tumor suppressor in human osteosarcoma by targeting HMGA1[J].Cell Physiol Biochem,2014,33(5):1329-1339. [5] Xiong Y,Zhang J,Song C.CircRNA ZNF609 functions as a competitive endogenous RNA to regulate FOXP4 expression by sponging miR-138-5p in renal carcinoma[J].Cell Physiol,2019,234(7):10646-10654. [6] Liu HS,Zheng RN,Guo LB,et al.Circular RNA circ_0000615 knockdown suppresses the development of nasopharyngeal cancer through regulating the miR-338-3p/FGF2 axis[J].Neoplasma,2020,67(5):1032-1041. [7] Niu Q,Dong Z,Liang M,et al.Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis[J].Exp Clin Cancer Res,2020,39(1):280-297. [8] Wu M,Kong C,Cai M,et al.Hsa_circRNA_002144 promotes growth and metastasis of colorectal cancer through regulating miR-615-5p/LARP1/mTOR pathway[J].Carcinogenesis,2021,42(4):601-610. [9] Gu Q,Hou W,Shi L,et al.Circular RNA ZNF609 functions as a competing endogenous RNA in regulating E2F transcription factor 6 through competitively binding to microRNA-197-3p to promote the progression of cervical cancer progression[J].Bioengineered,2021,12(1):927-936. [10] Liu Z,Pan HM,Xin L,et al.Circ-ZNF609 promotes carcinogenesis of gastric cancer cells by inhibiting miRNA-145-5p expression[J].Eur Rev Med Pharmacol Sci,2019,23(21):9411-9417. [11] Du S,Li H,Lu F,et al.Circular RNA ZNF609 promotes the malignant progression of glioma by regulating miR-1224-3p/PLK1 signaling[J].Cancer,2021,12(11):3354-3366. [12] Pan W,Pang J,Ji B,et al.RNA binding protein HuR promotes osteosarcoma cell progression via suppressing the miR-142-3p/HMGA1 axis[J].Oncol Lett,2018,16(2):1475-1482.
2022, Vol. 28 
冀公网安备13080202000677号