Abstract：Objective: To investigate the effects and possible mechanisms of suprapatellar-derived ASC autograft on knee inflammation and degenerative grade of cartilage in a mouse KOA model. Methods: ASC in the suprapatellar or infrapatellar region were isolated, ASC proliferation and fat-forming, osteogenic and cartilage-forming directed differentiation ability were measured respectively, the OA mouse model was replicated and cell transplantation was performed, and the GAG analysis and injury score of mouse knee tissue were examined. Results: In vitro experiments showed a significant increase in fat formation, osteogenesis and cartilage formation in the suprapatellar-derived ASC group compared to the infrapatellar-derived and infrapatellar-differentiated cell groups. In vivo experiments showed a significant increase in knee dimensions in all three groups after OA induction. hASC, SVF and PRP intervention groups all showed a significant decrease in increased knee volume compared to the control group, a significant increase in GAG content and a significant decrease in OA injury score in the hASC group. Conclusion: Human suprapatellar fat pad-derived ASC promotes cartilage regeneration by promoting effective endogenous cartilage formation in the knee joint in KOA mice.
白冰, 孙铭霞, 孙彦豹, 崔万勇, 张小雪, 王友. 脂肪源性干细胞在小鼠膝关节骨关节炎中的作用研究[J]. 河北医学, 2022, 28(10): 1659-1662.
BAI Bing, SU Mingxia, SUN Yanbao, et al. Effect of Adipose-Derived Stem Cells on Knee Osteoarthritis in Mice. HeBei Med, 2022, 28(10): 1659-1662.
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2022, Vol. 28 
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