Abstract：Objective: To investigate the changes of subcellular structure and expression profile of distal tissue in colorectal cancer liver metastasis. Methods: The structural changes of human liver were observed by electron microscope,and confirmed in a colorectal cancer liver metastasis mice model.Transcriptome profiling was used to explore the differential expressed genes in distal tissues in the mice model. Results: The subcellular structure of liver cells in distant tissue in both human and mouse liver metastases were obviously different from that of healthy liver cells.Transcriptome analysis showed that differentiated genes in distal tissues were enriched to pathways related to tumor. Conclusion: The morphological structure and gene expression of distal tissues were also affected by the liver metastasis.
[1] Arneth B.Tumor Microenvironment[J].Medicina (Kaunas),2019,56(1):15. [2] 樊嘉,顾晋,秦新裕,等.中国结直肠癌肝转移诊断和综合治疗指南(2020)[J].中国临床医学,2021,28(1):129-144 [3] 徐泽,徐杰.癌症治疗新概念与新方法[M].人民军医出版社,2011.163-164 [4] Bae K B,et al.An Animal model of colorectal cancer liver metastasis with a high metastasis rate and clonal dynamics[J].Anticancer Res,2020,40(6):3297-3306. [5] 刘复生.肿瘤病理学电镜图谱[M].北京医科大学.中国协和医科大学联合出版社,1995. [6] Nozawa R S,et al.Nuclear microenvironment in cancer:control through liquid-liquid phase separation[J].Cancer Sci,2020,111(9):3155-3163. [7] Denais C M,et al.Nuclear envelope rupture and repair during cancer cell migration[J].Science,2016,352(6283):353-358. [8] Sabharwal S S,P T Schumacker.mitochondrial ROS in cancer:initiators,amplifiers or an achilles' heel[J].Nat Rev Cancer,2014,14(11):709-721. [9] Zhou J F,et al.A Review of the roles of endoplasmic reticulum stress in cancer cell metastasis[J].Sichuan Da Xue Xue Bao Yi Xue Ban,2021,52(1):11-15. [10] Babour A,et al.A surveillance pathway monitors the fitness of the endoplasmic reticulum to control its inheritance[J].Cell,2010,142(2):256-269. [11] Azizidoost S,Ahmadzadeh A,Rahim F,et al.Hepatic metastatic niche:from normal to pre-metastatic and metastatic niche[J].Tumour Biol,2016,37(2):1493-503. [12] Yamakawa N,et al.Tumor budding and adjacent tissue at the invasive front correlate with delayed neck metastasis in clinical early-stage tongue squamous cell carcinoma[J].Surg Oncol,2019,119(3):370-378.
2022, Vol. 28 
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