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河北医学  2021, Vol. 27 Issue (9): 1409-1413    DOI: 10.3969/j.issn.1006-6233.2021.09.001
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新生VAP小鼠和肽素 IL-32 IL-17水平变化与不同程度肺炎的相关性分析
方平1, 王娅1, 吴迪1, 赵婧2
1.川北医学院, 四川 南充 637100
2.川北医学院附属医院儿科, 四川 南充 637000
Correlation Analysis of Neonatal VAP Mice and Copeptin IL-32 IL-17 Levels and Different Degrees of Pneumonia
FANG Ping, WANG Ya, WU Di, et al
North Sichuan Medical College, Sichuan Nanchong 637100, China
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摘要 目的: 探讨呼吸机相关性肺炎(VAP)的发病症状与影响因素,为临床诊断与治疗提供基础。方法: 将54只BALB/c雄性小鼠随机分为空白组、VAP轻症组(1×106cfu/L菌液)、VAP重症组(6×106cfu/L菌液),每组18只。除空白组外,其余各组小鼠进行机械通气24h与气管滴注铜绿假单胞菌构建VAP模型。模型构建后1d、3d、7d分别测定用力肺活量(FVC)、第1秒用力呼气量(FEV1),计算FEV1/FVC%值;ELISA法测定血清中Copeptin、IL-32、IL-17的含量;HE染色观察7d后肺脏中病理学改变;免疫组化法测定7d后肺组织NF-κB、TLR2与TLR4的表达水平;将肺功能与Copeptin、IL-32、IL-17的测定结果进行相关性分析,对Copeptin、IL-32、IL-17的结果进行ROC分析,评价VAP的影响因素。结果: VAP发生7d内,与空白组相比,轻症组与重症组动物的FVC、FEV1降低,血清中Copeptin、IL-32、IL-17升高(P<0.05);肺脏中炎性细胞、中性粒细胞数量,以及TRL2、TRL4、NF-κB蛋白的表达随VAP症状的加重而增多;相关性分析与ROC分析的结果显示:肺功能指标与Copeptin、IL-32、IL-17水平呈密切关联性,趋势呈负相关。结论: 呼吸机相关性肺炎的发病程度与Copeptin、IL-32、IL-17具有密切相关性,其机制可能与激活NF-κB通路介导炎症反应相关,从而降低肺功能。
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方平
王娅
吴迪
赵婧
关键词 呼吸机相关性肺炎和肽素IL-32IL-17    
AbstractObjective: To explore the symptoms and influencing factors of ventilator-associated pneumonia (VAP) to provide a basis for clinical diagnosis and treatment. Methods: 54 Balb/c mice were randomly divided into a control group, mild group (1×106 cfu/L), severe group (6×106 cfu/L) (n=18). Except for the control group, mice were mechanically ventilated for 24 hours and tracheal instillation of Pseudomonas aeruginosa to construct a VAP model. Forced vital capacity (FVC) and forced expiratory volume in the first second (FEV1) were measured at 1, 3, and 7 days after the model was constructed, respectively, and the value of FEV1/FVC% was calculated. The contents of Copeptin, IL-32 and IL-17 in serum were determined by ELISA. The staining was used to observe the histopathological changes of lung after 7 days. The protein expression levels of NF-κB, TLR2 and TLR4 in lung tissues were determined by immunohistochemical after 7 days. The lung function indexes were correlated with the levels of Copeptin, IL-32 and IL-17. The results of Copeptin, IL-32, IL-17 and the incidence of VAP were analyzed by ROC to evaluate the risk factors of VAP. Results: Within 7 days after the occurrence of VAP, compared with the control group, the FVC and FEV1 levels of the mild and severe groups were decreased, while the serum levels of Copeptin, IL-32 and IL-17 were increased (P<0.05); The number of inflammatory cells, neutrophils and the expression of TRL2, TRL4 and NF-κB protein in lung increased with the aggravation of VAP symptoms; Correlation analysis and ROC analysis showed that lung function indexes were closely correlated with the levels of Copeptin, IL-32, and IL-17, with a negative correlation trend. Conclusion: The incidence of ventilator-associated pneumonia is closely related to Copeptin, IL-32 and IL-17, and its mechanism may be related to the activation of NF-κB pathway to mediate inflammatory response, thereby reducing lung function.
Key wordsVentilator-associated pneumonia    Copeptin    IL-32    IL-17
    
基金资助:国家自然科学基金青年项目,(编号:81300528);四川省科技厅面上项目,(编号:2017JY0115);南充市科学技术和知识产权局项目,(编号:14A0058);川北医学院附属医院科技发展计划项目,(编号:2019ZD010)
通讯作者: 赵婧   
引用本文:   
方平, 王娅, 吴迪, 赵婧. 新生VAP小鼠和肽素 IL-32 IL-17水平变化与不同程度肺炎的相关性分析[J]. 河北医学, 2021, 27(9): 1409-1413.
FANG Ping, WANG Ya, WU Di, et al. Correlation Analysis of Neonatal VAP Mice and Copeptin IL-32 IL-17 Levels and Different Degrees of Pneumonia. HeBei Med, 2021, 27(9): 1409-1413.
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