Abstract：Objective: To analyze the effects of interference with TPX2 gene on the activity and radiotherapy sensitivity of HeLa cells. Methods: Cervical cancer Hela cell is cultured in vitro and divided into interference group, negative control group (NC group), blank control group (CON group), interference and CON group. Respectively the interference group had TPX2-siRNA interference plasmid; CON group had plasmid transfection, replacing the plasmid with PBS; NC group had blank load transfection. CCK 8 experimental detection of efficiency of cell proliferation, Transwell Chambers detection of invasion ability, clone forming experiments to detect radiation sensitivity were all conducted. Results: Compared with the CON group, the relative expression level of TPX2 mRNA, the relative expression level of TPX2 protein, and the cell invasion ability of the NC group did not change significantly (P>0.05); compared with the CON group and the NC group, the TPX2 of the interference group, the relative expression of mRNA, the relative expression of TPX2 protein, and the ability of cell invasion were all significantly reduced (P<0.05). Compared with the CON group, the cell viability of the NC group was not significantly different at each time point (P>0.05); compared with the CON group and the NC group, the culture time of the interference group was 48h, 72h, 96h. The cell viability at time was significantly reduced (P<0.05). Compared with the CON group, the proportion of cell cycles in the G0/G1, G2/M and S phases of the NC group did not change significantly (P>0.05); compared with the NC group, the S and G2/ The proportion of cells in M phase increased significantly, while the proportion of cells in G0/G1 phase decreased significantly (P<0.05). Compared with the CON group, the survival rate of the interference group cells at 4Gy, 6Gy, 8Gy, and 10Gy doses of radiotherapy decreased (P<0.05). Conclusion: Interference with TPX2 gene in cervical cancer Hela cells can effectively interfere with the expression of TPX2 gene and inhibit the cell activity of proliferation and migration of cervical cancer HeLa cells.Interference with TPX2 gene can effectively increase the radiotherapy sensitivity of cervical cancer HeLa cells and improve the curative effect of radiotherapy for cervical cancer.
祁小丽, 王玉婷, 杨杰. 干扰Hela细胞TPX2基因对细胞活性及其放疗敏感性的影响研究[J]. 河北医学, 2021, 27(8): 1239-1243.
QI Xiaoli, WANG Yuting, YANG Jie. Effect of Interference of TPX2 Gene on Cell Activity and Radiotherapy Sensitivity of Hela Cells. HeBei Med, 2021, 27(8): 1239-1243.
[1] Zhen X,Chen J,Zhong Z,et al.Deep convolutional neural network with transfer learning for rectum toxicity prediction in cervical cancer radiotherapy:a feasibility study[J].Phys Med Biol,2017,62(21):168~173. [2] Guo M,Huang E,Liu X,et al.Volumetric modulated arc therapy versus fixed-field intensity-modulated radiotherapy in radical irradiation for cervical cancer without lymphadenectasis:dosimetric and clinical results[J].Oncol Res Treat,2018,41(3):105~109. [3] Almeida V H D,Melo A,Meira D,et al.Radiotherapy modulates expression of EGFR,ERCC1 and p53 in cervical cancer[J].Braz Med Biol Res,2017,51(1):6822~6827. [4] Liu Q,Sun L,Bian L,et al.Relationship between cognitive differences in medical behaviors and degrees of anxiety of cervical cancer patients undergoing radiotherapy[J].Jilin Univ(Med Ed),2017,43(1):115~121. [5] Yin L,Lu S,Zhu J,et al.Ovarian transposition before radiotherapy in cervical cancer patients:functional outcome and the adequate dose constraint[J].Radiat Oncol,2019,14(1):100~106. [6] Zhang J Y,Hong D L.SU-F-T-397:Evaluating the impact of bladder filling status for the organs at risk dose distribution in cervical cancer patients with intensity modulated radiotherapy[J].Med Phys,2016,43(6):3554~3556. [7] Bao Y,Zhang S,Niyazi H,et al.Abstract LB-057:The expression level and prognostic value of PD-L1 and PD-1 in cervical carcinoma patients[J].Cancer Res,2017,77(13):57~59. [8] Yang K,Park W,Huh S J,et al.Clinical outcomes in patients treated with radiotherapy after surgery for cervical cancer[J].Radiat Oncol,2017,35(1):39~47. [9] 朱佳浩,顾科.宫颈癌放射敏感性预测在精准治疗中的研究现状及进展[J].中国临床实用医学,2018,9(6):74~76. [10] Wang P,Sun W,Wang L,et al.Correlations of p53 expression with transvaginal color doppler ultrasound findings of cervical cancer after radiotherapy[J].Buon,2018,23(3):769~775. [11] Zou J,Huang RY,Jiang FN,et al.Overexpression of TPX2 is associated with progression and prognosis of prostate cancer[J].Oncol Lett,2018,16(3):2823~2832.
2021, Vol. 27 
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