Abstract：Objective: To explore the clinical guiding significance through detecting the levels of peripheral blood regulatory T lymphocytes, serum interleukin-10 (IL-10) and specific IgE (sIgE) in bronchial asthma children allergic to Dermatophagoides pteronyssinus. Methods: From February 2018 to February 2020, 163 children with bronchial asthma who were allergic to Dermatophagoides pteronyssinus were selected as study subjects. According to the severity of the disease, they were divided into mild group (52 cases), moderate group (63 cases), and severe group (48 cases). Meanwhile, 50 healthy children of the same age were selected as the control group. The peripheral blood lymphocytes were isolated, and then cultured for 48 hours under the stimulation of Dermatophagoides pteronyssinus leachate. Flow cytometry was used to detect the percentages of adaptive regulatory T cells (iTreg) and type I regulatory T cells (Tr1). In addition, the lung function, serum IL-10, TGF-β and sIgE levels were compared among groups. Results: Comparison of the control group and the diseased group found that the severer the disease, the lower the forced expiratory volume in the first second (FEV1), the FEV1 to forced vital capacity (FVC) ratio (FEV1/FVC) and peak expiratory flow (PEF), the lower the proportions of iTreg and Tr1, showing statistically significant differences (P<0.05). Comparison of immune factor levels between the control group and the diseased group found that the severer the disease, the lower IL-10 and TGF-β levels (P<0.05), and the higher the sIgE level (P<0.05). Conclusion: The detection of lung function indexes, IL-10 secreting regulatory T lymphocytes and immune factors IL-10, TGF - β and SiGe levels in children with bronchial asthma not only helps to evaluate the severity of the disease, but also helps to provide reference for the prognosis of the disease in the process of treatment..
王汝琼, 李小明, 林道炯, 吴守业, 周琼花. 支气管哮喘患儿外周血调节性T淋巴细胞水平 IL-10 sIgE水平及其临床意义[J]. 河北医学, 2021, 27(7): 1127-1130.
WANG Ruqiong, LI Xiaoming, LIN Daojiong, et al. Levels and Clinical Significance of Peripheral Blood Regulatory T Lymphocytes IL-10 and SIgE in Children with Bronchial Asthma. HeBei Med, 2021, 27(7): 1127-1130.
2021, Vol. 27 
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