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河北医学  2021, Vol. 27 Issue (7): 1063-1069    DOI: 10.3969/j.issn.1006-6233.2021.07.002
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内源性Relaxin-1 Relaxin-3及其受体LGR7表达水平在小鼠心房颤动心肌纤维化中的作用
翁方中, 胡朝梁, 严骏, 戴伟, 周瑞祥
湖北省武汉市第一医院重症医学科, 湖北 武汉 430022
Role of Endogenous Relaxin-1 Relaxin-3 and Receptor LGR7 in Myocardial Fibrosis of Mice with Atrial Fibrillation
WENG Fangzhong, HU Chaoliang, YAN Jun, et al
Wuhan First Hospital, Hubei Wuhan 430022, China
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摘要 目的: 分析内源性松弛素(Relaxin,RLX)-1、Relaxin-3、松弛素受体(LGR7)表达水平在在心房颤动心肌纤维化中的作用。方法: 腹腔注射ISO构建心房颤动模型,40只C57B62小鼠随机分为空白对照组、模型组、模型组+RLX1、模型组+RLX3组,每组10只;模型组+RLX1、模型组+RLX3分别腹腔注射RLX1、RLX3,空白对照组、模型组注射等量生理盐水;一周后检测心肌胶原沉积纤维面积、羟脯氨酸含量,应用RT-PCR法、Western Blot法检测Relaxin 1、Relaxin 3、LGR7 mRNA及蛋白表达;免疫组化法检测α-SMA积分光密度(IOD),Western Blot法检测心肌α-SMA蛋白表达;Real-Time PCR、Western Blot法测定I型胶原蛋白(collagenⅠ)、Ⅲ型胶原蛋白(collagen Ⅲ)、基质金属蛋白酶-1(MMP-1)、MMP-2、MMP-9、MMP-13、金属蛋白酶组织抑制物-1(TIMP-1)mRNA及蛋白表达;RT-PCR法检测心肌血管紧张素Ⅱ(AngⅡ)、血管紧张素受体1(AT1R)、血管紧张素受体2(AT2R)、醛固酮(ALD)及盐皮质激素受体(MR)mRNA表达。结果: ①较空白对照组,模型组室间隔、右心室及左心室心肌胶原沉积纤维面积百分比,心肌羟脯氨酸含量均明显上升(P<0.05);与模型组比较,模型组+RLX1、模型组+RLX3室间隔、右心室及左心室心肌胶原沉积纤维面积百分比,心肌羟脯氨酸含量明显下降(P<0.05)。②与空白对照组比较,模型组Relaxin 1、Relaxin 3、LGR7 mRNA及蛋白表达明显下降(P<0.05);与模型组比较,模型组+RLX1、模型组+RLX3 Relaxin 1、Relaxin 3、LGR7 mRNA及蛋白表达明显上升(P<0.05)。③与空白对照组比较,模型组α-SMA IOD、α-SMA蛋白,collagenⅠ、collagenⅢ mRNA及蛋白表达明显上升(P<0.05),与模型组比较,模型组+RLX1、模型组+RLX3 α-SMA IOD、α-SMA蛋白、collagenⅠ、collagenⅢ mRNA及蛋白表达明显下降(P<0.05)。④与空白对照组比较,模型组MMP-1、MMP-2、MMP-9、MMP-13 mRNA及蛋白表达明显上升,TIMP-1 mRNA及蛋白表达明显下降(P<0.05);与模型组比较,模型组+RLX1、模型组+RLX3 MMP-1、MMP-2、MMP-9、MMP-13 mRNA及蛋白表达明显下降、TIMP-1 mRNA及蛋白明显上升(P<0.05)。⑤与空白对照组比较,模型组Ang Ⅱ mRNA、AT1R mRNA、AT2R mRNA、ALD mRNA、MR mRNA水平明显上升;与模型组比较,模型组+RLX1、模型组+RLX3 Ang Ⅱ mRNA、AT1R mRNA、AT2R mRNA、ALD mRNA、MR mRNA明显下降。结论: 心房颤动小鼠心肌存在纤维化病理改变,且内源性Relaxin-1、Relaxin-3、LGR7低表达,Relaxin或可通过AngⅡ及ALD系统拮抗心肌纤维化。
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关键词 心房颤动心肌纤维化松弛素-1松弛素-3松弛素受体    
AbstractObjective: To analyze the role of endogenous relaxin (RLX)-1, relaxin-3 and relaxin receptor (LGR7) in myocardial fibrosis of mice with atrial fibrillation. Methods: Atrial fibrillation model was constructed by intraperitoneal injection of ISO. 40 C57B62 mice were randomly divided into blank control group, model group, model group + RLX1 and model group + RLX3 group with 10 mice in each group. The model group + RLX1 and model group + RLX3 were intraperitoneally injected with RLX1 and RLX3, respectively. The blank control group and model group were injected with the same volume of normal saline. A week later, the area of myocardial collagen deposition and the content of hydroxyproline were detected. RT-PCR and Western Blot were used to detect the mRNA and protein expression of relaxin 1, relaxin 3 and LGR7. Immunohistochemical method was used to detect α-SMA integral optical density (IOD), and Western Blot was used to detect the expression of α-SMA protein in myocardial tissues. Real-time PCR and Western Blot were used to detect the mRNA and protein expression of collagenⅠ, collagen III, matrix metalloproteinase-1 (MMP-1), MMP-2, MMP-9, MMP-13, and tissue inhibitor of metalloproteinase-1 (TIMP-1). Meanwhile, RT-PCR method was used to detect the mRNA expression of myocardial angiotensin II (AngII), angiotensin receptor 1 (AT1R), angiotensin receptor 2 (AT2R), aldosterone (ALD) and mineralocorticoid receptor (MR). Results: ①Compared with the blank control group, the percentages of myocardial collagen deposition areas in ventricular septum, right ventricle and left ventricle, and the content of hydroxyproline were significantly increased in the model group (P<0.05). Compared with the model group, the percentages of myocardial collagen deposition areas in ventricular septum, right ventricle and left ventricle, and the content of hydroxyproline were significantly reduced in the model group+RLX1 and model group+RLX3 (P<0.05). ②Compared with the blank control group, the mRNA and protein expression of relaxin 1, relaxin 3 and LGR7 was significantly reduced in the model group (P<0.05). Compared with the model group, the mRNA and protein expression of relaxin 1, relaxin 3 and LGR7 was significantly increased in model group +RLX1 and model group+RLX3 (P<0.05). ③Compared with the blank control group, α-SMA IOD, α-SMA protein, the mRNA and protein expression of collagenⅠ and collagenⅢ were significantly increased in the model group (P<0.05). Compared with the model group, α-SMA IOD, α-SMA protein, the mRNA and protein expression of collagenⅠ and collagenⅢ were significantly reduced in the model group+RLX1 and the model group+RLX3 (P<0.05). ④Compared with the blank control group, the mRNA and protein expression of MMP-1, MMP-2, MMP-9 and MMP-13 was significantly increased, while the expression of TIMP-1 mRNA and protein was significantly reduced in the model group (P<0.05). Compared with the model group, the mRNA and protein expression of MMP-1, MMP-2, MMP-9 and MMP-13 was significantly decreased, while the expression of TIMP-1 mRNA and protein was significantly increased in the model group+RLX1 and model group+RLX3 (P<0.05). ⑤Compared with the blank control group, the levels of Ang Ⅱ mRNA, AT1R mRNA, AT2R mRNA, ALD mRNA, and MR mRNA were significantly increased in the model group. Compared with the model group, the levels of Ang Ⅱ mRNA, AT1R mRNA, AT2R mRNA, ALD mRNA, and MR mRNA were significantly reduced in the model group+RLX1 and model group+RLX3. Conclusion: The pathological changes of myocardial fibrosis are observed in the heart of the AF mice, and the endogenous relaxin-1, relaxin-3 and LGR7 are low expression, and relaxin could be antagonized by AngII and ALD system.
Key wordsAtrial fibrillation    Myocardial fibrosis    Relaxin-1    Relaxin-3    Relaxin receptor
    
基金资助:湖北省武汉市卫生健康委科研项目,(编号:WX20D92)
引用本文:   
翁方中, 胡朝梁, 严骏, 戴伟, 周瑞祥. 内源性Relaxin-1 Relaxin-3及其受体LGR7表达水平在小鼠心房颤动心肌纤维化中的作用[J]. 河北医学, 2021, 27(7): 1063-1069.
WENG Fangzhong, HU Chaoliang, YAN Jun, et al. Role of Endogenous Relaxin-1 Relaxin-3 and Receptor LGR7 in Myocardial Fibrosis of Mice with Atrial Fibrillation. HeBei Med, 2021, 27(7): 1063-1069.
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