Transfection of STAT3 Overexpression Plasmid Mediated by Recombinant Lentivirus Promotes Differentiation of Bone Marrow Mesenchymal Stem Cells into Neural Cells in Fetal Rats with Spina Bifida Aperta
JIANG Mingyu, REN Mingyong, GAO Ying, et al
The First Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150001, China
Abstract：Objective: To observe the action of recombinant lentivirus mediated STAT3 overexpression plasmid transfected into bone marrow mesenchymal stem cells on neural cell trans-differentiation in spinal cord tissue of fetal rats with spina bifida aperta. Methods: The fetal rat model of spina bifida aperta was established by retinoic acid and treated by microsurgical injection of stem cells. They were divided into blank control group (12 cases),negative control group (10 cases) and experimental group (11 cases). OD value of BMSCs viability was determined by CCK-8 kit. The expression of STAT3,pSTAT3,nerve markers (GFAP,NSE,NF and nestin) and apoptosis related factors (Caspase-8 and Bcl-2) were tested by the method of real-time PCR and Western blot. Results: OD value in experimental group was the highest at 8 hours after transplantation by CCK-8 kit,and then gradually decreased,which was statistically significant compared with blank control group and negative control group (P<0.05). There was no statistical difference in OD peak time and value between blank control group and negative control group. The expression levels of pSTAT3,GFAP,NSE,NF and nestin in experimental group were remarkably higher than those in blank control group and negative control group (P<0.05),but STAT3 expression in experimental group were statistically decreased (P<0.05). The relative expression levels of Caspase-8 and Bcl-2 in experimental group were tremendously lower than those in blank control group and negative control group (P<0.05). Conclusion: Transfection of recombinant lentivirus mediated STAT3 overexpression plasmid with BMSCs can improve the efficiency of transforming into neural cells and provide new seed cells for the treatment of congenital spina bifida aperta.
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