miR-29a miR-126a-5p miR-21-3p在重症急性胰腺炎中的表达及相关性研究

doi:10.3969/j.issn.1006-6233.2021.12.012

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摘要 目的: 探究微小RNA-29a(miR-29a)、微小RNA-126a-5p(miR-126a-5p)、微小RNA-21-3p(miR-21-3p)在重症急性胰腺炎中的表达及相关性研究。方法: 选取2020年3月至2021年3月于我院收治的急性胰腺炎患者82例作为观察组,其中轻症急性胰腺炎患者43例,重症急性胰腺炎患者39例,另外选取同期40例正常体检者作为对照组。实时荧光定量法检测两组受试者miR-29a、miR-126a-5p、miR-21-3p表达,急性生理与慢性健康评估(APACHEⅡ)、RANSON评分系统对患者的严重程度进行评分,Pearson相关性对miR-29a、miR-126a-5p、miR-21-3p相关性进行分析,探究miR-29a、miR-126a-5p、miR-21-3p在重症急性胰腺炎病症中的相关性。结果: 与对照组相比,观察组患者miR-29a、miR-21-3p表达水平上升,miR-126a-5p表达水平下降,具有统计学意义(P<0.05)。与轻症急性胰腺炎患者相比,重症急性胰腺炎患者miR-29a、miR-21-3p表达水平较低,miR-126a-5p表达水平较高,具有统计学意义(P<0.05)。随着重症急性胰腺炎患者病情加重,miR-29a、miR-21-3p表达水平上升,miR-126a-5p表达水平下降,具有统计学意义(P<0.05)。本文结果显示,miR-29a、miR-126a-5p呈现出负相关(r=-0.447;P=0.003);miR-29a、miR-21-3p呈现出正相关(r=0.534;P=0.001);miR-126a-5p、miR-21-3p呈现出负相关(r=-0.517;P=0.001)。结论: miR-29a、miR-21-3p在重症急性胰腺炎患者血清中呈现高表达,miR-126a-5p在重症急性胰腺炎患者血清中呈现低表达,三者可作为重症急性胰腺炎的检测指标。

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	张倩倩
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	吕杰

关键词 ：重症急性胰腺炎, 微小RNA-29a, 微小RNA-21-3p, 微小RNA-126a-5p

Abstract：Objective: To explore the expression and correlation of microRNA-29a (miR-29a), microRNA-126a-5p (miR-126a-5p) and microRNA-21-3p (miR-21-3p) in severe acute pancreatitis. Methods: A total of 82 patients with acute pancreatitis in our hospital from March 2020 to March 2021 were selected as the observation group, including 43 patients with mild acute pancreatitis, 39 patients with severe acute pancreatitis, and 40 normal physical examinees in the same period were selected as the control group. The expressions of miR-29a, mir-126a-5p and mir-21-3p were detected by real-time fluorescence quantitative method. The severity of patients was evaluated by acute physiology and chronic health assessment (APACHE II) and Ranson scoring system. Pearson correlation was used to analyze the correlation of miR-29a, mir-126a-5p and mir-21-3p. Results: Compared with the control group, the expression levels of miR-29a and miR-21-3p in the observation group increased, and the expression levels of miR-126a-5p decreased, with statistical significance (P<0.05). Compared with patients with mild acute pancreatitis, the expression levels of miR-29a and miR-21-3p reduce in patients with severe acute pancreatitis, and the expression levels of miR-126a-5p rise, with statistical significance (P<0.05). With the exacerbation of severe acute pancreatitis, the expression levels of miR-29a and miR-21-3p increased, and the expression levels of miR-126a-5p decreased, with statistical significance (P<0.05). The results show that the negative phase of miR-29a is related to mir-126a-5p (r=-0.447; P = 0.003); the positive correlation of miR-29a with mir-21-3p (r=0.534; P = 0.001);the negative phase of mir-126a-5p was related to mir-21-3p (r=-0.517; P = 0.001). Conclusion: MiR-29a and miR-21-3p are highly expressed in the serum of patients with severe acute pancreatitis, and miR-126a-5p is low expressed in the serum of patients with severe acute pancreatitis, and abnormal levels of miR-29a, miR-126a-5p and miR-21-3p were risk factors for severe acute pancreatitis. It can be used as an indicator for the detection of severe acute pancreatitis.

Key words： Severe acute pancreatitis MicroRNA-29a MicroRNA- 21-3p MicroRNA-126-a-5p

基金资助:陕西省社会发展科技攻关项目,(编号:2020KJ-155)

引用本文:

张倩倩, 王成立, 伏添, 吕杰. miR-29a miR-126a-5p miR-21-3p在重症急性胰腺炎中的表达及相关性研究[J]. 河北医学, 2021, 27(12): 1991-1995.
ZHANG Qianqian, WANG Chengli, Fu Tian, et al. The Expression and Correlation of MiR-29a MiR-126a-5p and MiR-21-3p in Severe Acute Pancreatitis. HeBei Med, 2021, 27(12): 1991-1995.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.12.012 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I12/1991

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