温阳活血汤治疗骨转移癌作用机制探讨

doi:10.3969/j.issn.1006-6233.2021.11.032

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摘要 目的: 通过网络药理学的方法研究温阳活血汤治疗骨转移癌的作用机制。方法: 利用TCMSP、TCMID和TCM Database@Taiwan数据库以及文献检索的方式筛选药物主要有效成分,利用Swiss Target Prediction和TCMSP数据库检索温阳活血汤潜在治疗靶点,通过DisGeNet和Drugbank检索骨转移癌疾病基因,并将药物作用靶点和疾病基因进行映射,通过Cytoscape3.6.0软件进行可视化并筛选出核心基因,对潜在治疗靶点进行GO和KEGG通路分析。结果: 温阳活血汤的主要有效成分有109个,包含374个靶蛋白。骨转移癌的疾病靶点有773个,其中105个是温阳活血汤治疗骨转移癌的潜在靶点。通过GO和KEGG通路分析发现温阳活血汤治疗骨转移癌是通过HIF-1、TNF、VEGF等信号通路发挥抗癌、抑制骨转移和免疫系统调节的作用机制。温阳活血汤治疗骨转移癌的关键基因为VEGFA、AKT1、IL6、TP53、ALB、CXCL8、CASP3、TNF、EGFR、EGF和MAPK3等。结论: 通过网络药理学发现温阳活血汤治疗骨转移癌的作用机制涉及多靶点、多通路,可能与抗癌、抑制骨转移、调节免疫系统等相关。

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关键词 ：骨转移癌, 网络药理学, 温阳活血汤, 作用机制

Abstract：Objective: To study the mechanism of Wenyang Huoxue Decoction in the treatment of bone metastatic carcinoma by network pharmacology. Methods: TCMSP, TCMID and TCM Database@Taiwan databases and literature search were used to screen the main effective components of drugs. Swiss Target and TCMSP databases were used to search the potential therapeutic targets of Wenyang Huoxue Decoction. DisGeNet and Drugbank databases were used to search the genes of bone metastatic carcinoma. Drug action targets and disease genes were mapped. Cytoscape 3.6.0 software was used to visualize and screen out the core genes. GO and KEGG pathway analysis was performed on potential therapeutic targets. Results: There were 109 main active ingredients in Wenyang Huoxue Decoction, which contained 374 target proteins. There are 773 disease targets of bone metastatic carcinoma, of which 105 are potential targets of Wenyang Huoxue Decoction for the treatment of bone metastatic carcinoma. Through GO and KEGG pathway analysis, it was found that Wenyang Huoxue Decoction exerted anti-cancer, inhibiting bone metastasis and immune system regulation mechanism through HIF-1, TNF, VEGF and other signaling pathways in the treatment of bone metastatic carcinoma. The key genes of Wenyang Huoxue Decoction in treating bone metastatic carcinoma are VEGFA, AKT1, IL6, TP53, ALB, CXCL8, CASP3, TNF, EGFR, EGF and MAPK3 and so on. Conclusion: Through network pharmacology, we found that the mechanism of Wenyang Huoxue Decoction in treating bone metastatic carcinoma involves multiple targets and pathways, which may be related to anti-cancer, inhibition of bone metastasis, regulation of immune system, etc.

Key words： Bone metastatic carcinoma Network pharmacology Wenyang huoxue decoction Mechanism of action

基金资助:中国自然基金面上项目,(编号:81973640);中国中医科学院望京医院院级课题,(编号:WJYY2020-26)

通讯作者: 芦殿荣

引用本文:

杨梦霞, 毛昀, 周磊, 朱世杰, 王宁军, 芦殿荣. 温阳活血汤治疗骨转移癌作用机制探讨[J]. 河北医学, 2021, 27(11): 1922-1928.
YANG Mengxia, MAO Yun, et al. Study on the Mechanism of Wenyang Huoxue Decoction in the Treatment of Bone Metastatic Carcinoma. HeBei Med, 2021, 27(11): 1922-1928.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.11.032 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I11/1922

[1] 王骁,陈丽,刘广杰,等.骨转移癌疼痛的治疗进展[J].中国全科医学,2020,23(12):1572~1575.
[2] Huismanim,VAN Denbosch M A,Wijlemans J W,et al.Effectiveness of reirradiation for painful bone metastases:a systematic review and meta-analysis[J].Int Radiat Oncol Biol Phys,2012,84(1):8~14.
[3] 李志明,胡凯文,范毅南,等.补肾化痰法治疗骨转移癌疗效及安全性的Meta分析[J].中医学报,2020,35(6):1350~1356.
[4] 杨鹭,张程程,王中奇.肿瘤骨转移中医病因病机研究进展[J].中医文献杂志,2019,37(3):58~60.
[5] Weidle UH,Birzele F,Kollmorgen G,et al.Molecular mechanisms of bone metastasis[J].Cancer Genomics Proteomics,2016,13(1):1~12.
[6] 毛昀,陈峥,褚雪镭,等.国医大师朱良春治疗骨转移临证经验[J].湖南中医药大学学报,2020,40(9):1101~1105.
[7] Li Z,Zhang J,Ren X,et al.The mechanism of quercetin in regulating osteoclast activation and the PAR2/TRPV1 signaling pathway in the treatment of bone cancer pain[J].Int Clin Exp Pathol,2018,11(11):5149~5156.
[8] Yuan L,ZhangF,ShenM,et al. Phytosterols suppress phagocytosis and inhibit inflammatory mediators via ERK pathway on LPS-triggered inflammatory responses in RAW264.7 macrophages and the correlation with their structure[J].Foods,2019,8(11):582.
[9] 王颖,洪铭岩,陈梅,等.木犀草素对宫颈癌细胞增殖与迁移能力影响机制探讨[J].中华肿瘤防治杂志,2020,27(17):1357~1362.
[10] Liang H,Xiao J,Zhou Z,et al.Hypoxia induces miR-153 through the IRE1α-XBP1 pathway to fine tune the HIF1α/VEGFA axis in breast cancer angiogenesis[J].Oncogene,2018,37(15):1961~1975.
[11] Yang S,Wang H,Qin C,et al.Up-regulation of CXCL8 expression is associated with a poor prognosis and enhances tumor cell malignant behaviors in liver cancer[J].Biosci Rep,2020,40 (8):1169.
[12] HarrisAL.Hypoxia~a key regulatory factor in tumour growth[J].Nat Rev Cancer,2002,2(1):38~47.
[13] Zheng LW,Wang WC,Mao XZ,et al.TNF-α regulates the early development of avascular necrosis of the femoral head by mediating osteoblast autophagy and apoptosis via the p38 MAPK/NF-κB signaling pathway[J].Cell Biol Int,2020,44(9):1881~1889.
[14] Ma W,Chen X,Wu X,et al.Long noncoding RNA SPRY4-IT1 promotes proliferation and metastasis of hepatocellular carcinoma via mediating TNF signaling pathway[J].Cell Physiol,2020,235(11):7849~7862.
[15] Jimenez-Dalmaroni MJ,Gerswhin ME,Adamopoulos IE.The critical role of toll-like receptors:from microbial recognition to autoimmunity:a comprehensive review[J].Autoimmun Rev,2016,15(1):1~8.