Abstract：Objective: To investigate the effect of Dahuang Mudan Decoction on lipopolysaccharide (LPS)-induced pyrosis of intestinal epithelial cells and the influence on nucleotide binding oligomerization domain-like receptor family pyrin domain containing 3/caspase-1 (NLRP3/caspase-1) pathway. Methods: Intestinal epithelial cells were randomly divided into control group, LPS group and rhubarb Mudan Decoction low, medium and high dose groups (2.52, 5.04 and 7.56 g / kg). Blank serum was added to the control group, LPS and blank serum were added to the LPS group, and LPS and medicated serum were added to the rhubarb Mudan Decoction group. The activity of intestinal epithelial cells was detected by thiazole blue (MTT), the apoptosis of intestinal epithelial cells was detected by flow cytometry, the expression levels of NLRP3, caspase-1 and gsdmd-c were detected by Western blot (WB), the expression of NLRP3 in cells was detected by immunofluorescence, and the pro-inflammatory factor interleukin (IL) - 1 was detected by enzyme-linked immunosorbent assay (ELISA) β And IL-18. Results: Compared with those in the control group, the cell activity of LPS group was significantly decreased, the apoptotic index increased significantly, the protein expression of NLRP3, caspase-1 and GSDMD-C, and the contents of IL-1β and IL-18 were significantly increased (all P<0.05); compared with those in LPS group, the cell activity of Dahuang Mudan Decoction group was significantly increased, the apoptotic index decreased significantly, the protein expression of NLRP3, caspase-1 and GSDMD-C, and the contents of IL-1β and IL-18 were significantly decreased (all P<0.05). Conclusion: Dahuang Mudan Decoction can inhibit NLRP3/caspase-1 pathway and reduce LPS-induced pyrosis of intestinal epithelial cells.
[1] 卫威,余治高,宋振举.Gpr174对脓毒症肠道菌群和代谢的影响[J].中华急诊医学杂志,2021,30(4):443~448. [2] Pfalzgraff A,Weindl G.Intracellular lipopolysaccharide sensing as a potential therapeutic target for sepsis[J].Trends Pharmacol Sci,2019,40(3):187~197. [3] Tang YS,Zhao YH,Zhong Y,et al.Neferine inhibits LPS-ATP-induced endothelial cell pyroptosis via regulation of ROS/NLRP3/Caspase-1 signaling pathway[J].Inflamm Res,2019,68(9):727~738. [4] 高金鸟,黄秀锦,李芳,等.五味消毒饮合大黄牡丹皮汤加减联合西药治疗急性盆腔炎的临床疗效观察[J].中国中医基础医学杂志,2017,23(10):1422~1426. [5] Bein A,Zilbershtein A,Golosovsky M,et al.LPS induces hyper-permeability of intestinal epithelial cells[J].Cell Physiol,2017,232(2):381~390. [6] Liu HH,Zhang MW,Guo JB,et al.Procalcitonin and C-reactive protein in early diagnosis of sepsis caused by either gram-negative or gram-positive bacteria[J].Ir Med Sci,2017,186(1):207~212. [7] Kumar M,Leoncoria A,Cornick S,et al.Increased intestinal permeability exacerbates sepsis through reduced hepatic SCD-1 activity and dysregulated iron recycling[J].Nat Commun,2020,11(1):483. [8] Hersoug LG,Moller P,Loft S,et al.Role of microbiota-derived lipopolysaccharide in adipose tissue inflammation,adipocyte size and pyroptosis during obesity[J].Nutr Res Rev,2018,31(2):153~163. [9] Liang HH,Liu YF.Gasdermins pore cell membrane to pyroptosis[J].Sci China Life Sci,2016,59(10):1090~1092. [10] Zhang CP,Zhao CQ,Chen XY,et al.Induction of ASC pyroptosis requires gasdermin D or caspase-1/11-dependent mediators and IFNβ from pyroptotic macrophages[J].Cell Death Dis,2020,11(6):470. [11] Wen B,Ma C,Zhang Q,et al.Mechanism of qingre yangyin chushi pill in the treatment of gout based on NLRP3/GSDMD pyroptosis pathway[J].Drug Combinat Ther,2020,2(2):54~66. [12] 熊云昭,王萱,常奕,等.化瘀解毒中药抑制梗阻性肾病巨噬细胞浸润减轻炎性损伤的作用[J].天然产物研究与开发,2019,31(2):211~215. [13] 张志飞,方玉明,夏正新.大黄牡丹汤加味保留灌肠联合低分子肝素钙治疗重症急性胰腺炎的临床疗效及对患者肠黏膜屏障功能和炎症因子的影响[J].河北中医,2019,41(2):94~98. [14] 宋冰,汪永锋,余四九,等.基于TLR4/MYD88信号通路探讨大黄牡丹汤对急性胰腺炎大鼠模型的保护作用[J].中国实验动物学报,2021,29(1):17~26.
2021, Vol. 27 
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