PTEN和Notch1在非小细胞肺癌组织中表达及临床意义

doi:10.3969/j.issn.1006-6233.2021.10.011

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摘要 目的:探讨第10号染色体同源丢失性磷酸酶张力蛋白基因(PTEN)和Notch受体1(Notch1)在非小细胞肺癌(NSCLC)组织中的表达以及临床意义。方法:收集本院2016年1月至2017年12月收治的67例NSCLC患者癌组织标本,同期选取25例经组织病理学证实为正常肺组织标本为对照组。免疫组化法检测NSCLC组织中蛋白PTEN、Notch1的表达,分析二者与NSCLC患者临床资料间的关系。对NSCLC患者进行随访,随访时间36个月。Kendall's tau-b法分析NSCLC组织中PTEN、Notch1的相关性;Kaplan-Meier生存曲线分析PTEN、Notch1与NSCLC患者预后之间的关系;COX回归分析影响NSCLC患者预后的因素。结果:与正常肺组织比较,NSCLC组织中蛋白PTEN阳性表达显著降低,蛋白Notch1阳性表达率显著升高,差异具有统计学意义(P<0.05)。与未发生淋巴结转移和TNM I+II期的NSCLC患者比较,发生淋巴结转移和TNM III期的NSCLC患者PTEN阳性表达率显著降低,Notch1阳性表达率显著升高,差异具有统计学意义(P<0.05)。NSCLC组织中蛋白PTEN、Notch1表达呈负相关(r=-0.356,P<0.05)。PTEN阳性表达组患者累积生存率(63.0%)高于阴性表达组(44.6%);Notch1阳性表达组患者累积生存率(43.1%)低于阴性表达组(63.0%)。多因素COX分析表明,TNM分期、Notch1阳性表达、PTEN阴性表达是影响NSCLC患者预后的危险因素(P<0.05)。结论:PTEN、Notch1在NSCLC组织和正常肺组织中的表达比较具有显著差异性,且与NSCLC患者淋巴结转移和TNM分期有关,可能是NSCLC发生发展中的重要因素。

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关键词 ：非小细胞肺癌, 第10号染色体同源丢失性磷酸酶张力蛋白基因, Notch受体1

Abstract：Objective: To investigate the expression and clinical significance of gene of phosphate and tension homology deleted on chromosome ten (PTEN) and Notch receptor 1 (Notch1) in non-small cell lung cancer (NSCLC) tissues. Methods: 67 cases of cancer tissue specimens from NSCLC patients in our hospital from January 2016 to December 2017 were collected. At the same time, twenty-five cases of lung tissue specimens confirmed by histopathology were selected as the control group. Immunohistochemical method was used to detect the expression of PTEN and Notch1 in NSCLC tissues, and to analyze the relationship between the two and the clinical data of NSCLC patients. The patients with NSCLC were followed up for 36 months. Kendall's tau-b method was used to analyze the correlation between PTEN and Notch1 in NSCLC tissues; Kaplan-Meier survival curve was used to analyze the relationship between PTEN, Notch1 and the prognosis of NSCLC patients; COX regression was used to analyze the factors affecting the prognosis of NSCLC patients. Results: Compared with normal lung tissue, the positive expression of PTEN in NSCLC tissue was significantly reduced, and the positive expression rate of Notch1 was significantly increased, the difference was statistically significant (P<0.05). Compared with NSCLC patients without lymph node metastasis and with TNM stage I+II, NSCLC patients with lymph node metastasis and TNM stage III had a significantly lower the positive expression rate of PTEN, and a significantly higher positive expression rate of Notch1, the difference was statistically significant (P<0.05). The expression of PTEN and Notch1 in NSCLC tissue was negatively correlated (r=-0.356, P<0.05). The cumulative survival rate of patients in the PTEN positive expression group (63.0%) was higher than that in the negative expression group (44.6%); the cumulative survival rate of patients in the Notch1 positive expression group (43.1%) was lower than that in the negative expression group (63.0%). Multivariate COX analysis showed that TNM staging, positive expression of Notch1, and negative expression of PTEN were risk factors affecting the prognosis of NSCLC patients (P<0.05). Conclusion: The expression of PTEN and Notch1 in NSCLC tissues and normal lung tissues are significantly different, they are related to lymph node metastasis and TNM staging in NSCLC patients, and may be important factors in the occurrence and development of NSCLC.

Key words： Non-small cell lung cancer Gene of phosphate and tension homology deleted on chromosome ten Notch receptor 1

基金资助:河北省2019年医学科学研究课题计划,(编号:20191015);河北省2019年医学科学研究课题计划,(编号:20191011)

通讯作者: 池菲

引用本文:

张媛媛, 马静, 何会娜, 郭银, 李香兰, 池菲. PTEN和Notch1在非小细胞肺癌组织中表达及临床意义[J]. 河北医学, 2021, 27(10): 1637-1642.
ZHANG Yuanyuan, MA Jing, HE Huina, et al. Expression and the Clinical Significance of PTEN and Notch1 in Non-small cell Lung Cancer. HeBei Med, 2021, 27(10): 1637-1642.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.10.011 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I10/1637

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