CIAPIN1通过抑制ERK1/2途径对膀胱尿路上皮癌增殖和转移的作用研究

doi:10.3969/j.issn.1006-6233.2021.01.012

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摘要 目的: 探究CIAPIN1在膀胱尿路上皮癌中的作用及其可能的机制。方法: 以膀胱尿路上皮癌细胞系J82、T24、UM-UC-3和人膀胱上皮永生化细胞SV-HUC-1为研究对象,qRT-PCR检测细胞CIAPIN1 mRNA表达水平;Western blot检测细胞CIAPIN1、p-ERK1/2和ERK1/2 蛋白表达水平;5-乙炔基-2'-脱氧尿苷(EdU)标记实验检测细胞增殖;细胞划痕实验检测细胞迁移;Transwell实验检测细胞侵袭。结果: 与SV-HUC-1组相比,T24、UM-UC-3和J8两组细胞中CIAPIN1 mRNA和蛋白表达水平均明显升高(P<0.05),其中,T24组细胞变化最为明显,因此选取T24进行后续实验。与NC组相比,si-CIAPIN1组EdU阳性细胞数、细胞迁移距离和侵袭细胞数明显降低(P<0.05),p-ERK1/2 / ERK1/2蛋白表达水平明显升高(P<0.05);与si-CIAPIN1组相比,si-CIAPIN1+ U0126组EdU阳性细胞数、迁移距离和侵袭细胞数均明显升高(P<0.05),p-ERK1/2 / ERK1/2蛋白表达水平明显降低(P<0.05)。结论: CIAPIN1通过抑制ERK1/2途径磷酸化促进膀胱尿路上皮癌细胞的增殖和转移。

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关键词 ： CIAPIN1, 膀胱尿路上皮癌, 增殖, 转移, ERK1/2途径

Abstract：Objective: To explore the role and possible mechanism of CIAPIN1 in bladder urothelial carcinoma. Methods: Bladder urothelial cancer cell lines J82, T24, UM-UC-3 and human bladder epithelial immortalized cells SV-HUC-1 were used as the research objects. qRT-PCR was used to detect the expression level of CIAPIN1 mRNA in the cells; Western blot was used to detect the expression levels of CIAPIN1 p-ERK1/2 and ERK1/2 protein in cells; 5-ethynyl-2'-deoxyuridine (EdU) labeling test was used to detect cell proliferation; cell scratch test was used to detect cell migration; Transwell test was used to detect cell invasion. Results: Compared with SV-HUC-1 group, the expression levels of CIAPIN1 mRNA and protein in cells of T24, UM-UC-3 and J82 groups were significantly increased (P<0.05). Cells in T24 group had the most obvious changes, so T24 cells were selected for subsequent experiments. Compared with the NC group, the number of EdU-positive cells, cell migration distance, and invasive cells in the si-CIAPIN1 group were significantly reduced (P<0.05), and protein expression level of p-ERK1/2/ERK1/2 was significantly increased (P<0.05) ; Compared with the si-CIAPIN1 group, the number of EdU positive cells, migration distance, and number of invasive cells in the si-CIAPIN1+ U0126 group were significantly increased (P<0.05), and the protein expression level of p-ERK1/2 / ERK1/2 was significantly reduced ( P<0.05). Conclusion: CIAPIN1 promotes the proliferation and metastasis of bladder urothelial cancer cells by inhibiting ERK1/2 pathway phosphorylation.

Key words： CIAPIN1 Bladder urothelial carcinoma Proliferation Metastasis ERK1/2 pathway

基金资助:河北省保定市科技计划项目,(编号:1951ZF042)

通讯作者: 彭洪涛

引用本文:

袁晓菲, 彭洪涛, 马建锋, 邵丽军, 王海江, 张朝华, 张定元. CIAPIN1通过抑制ERK1/2途径对膀胱尿路上皮癌增殖和转移的作用研究[J]. 河北医学, 2021, 27(1): 50-55.
YUAN Xiaofei, PENG Hongtao, MA Jianfeng, et al. Study on Effect of CIAPIN1 on Proliferation and Metastasis of Bladder Urothelial Carcinoma by Inhibiting ERK1/2 Pathway. HeBei Med, 2021, 27(1): 50-55.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.01.012 或 http://www.hbyxzzs.cn/CN/Y2021/V27/I1/50

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