Abstract：Objective: To investigate the role of AmpC and ompk35 in imipenem induced resistance of Klebsiella pneumoniae. Methods: A total of 200 strains of Klebsiella pneumoniae isolated and cultured in our hospital from January 2015 to January 2020 were collected, among which 50 strains were resistant to imipenem (group A), 150 strains were not resistant to imipenem (group B), the gene expression of AmpC and ompk35 was detected by PCR, and the protein expression of AmpC and ompk35 was detected by Western blot. Results: The relative expression of AmpC gene and protein in group A were (1.658±0.122) and (1.501±0.106), which were significantly higher than that in group B (P < 0.05); The relative expression of ompk35 gene and protein in group A were (0.712±0.110) and (0.611±0.107), which were significantly lower than that in group B (P < 0.05); The correlation between AmpC gene and ompk35 gene was negative (r=-0.329, P < 0.05); the correlation between AmpC protein and ompk35 protein was negative (r=-0.435,p＜0.05). Conclusion: The expression of AmpC in imipenem resistant Klebsiella pneumoniae increase, and the expression of ompk35 decrease. They may play an important role in the mechanism of drug resistance.
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2021, Vol. 27 
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