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河北医学  2021, Vol. 27 Issue (1): 1-4    DOI: 10.3969/j.issn.1006-6233.2021.01.001
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MicroRNA-193a 通过靶向 WT1抑制肝细胞癌发生的机制研究
刘亚琪1, 郑承红2
1.湖北省襄阳市中医医院肿瘤科, 湖北 襄阳 441000
2.湖北省武汉市中医医院, 湖北 武汉 430065
Mechanism of MicroRNA-193a Suppressing the Tumorigenesis of Hepatocellular Carcinoma Through Targeting WT1
LIU Yaqi, et al
Xiangyang Hospital of Traditional Chinese Medicine, Hubei Xiangyang 441000, China
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摘要 目的: 通过检测miR-193a在肝细胞癌(Hepatocellular carcinoma,HCC) 组织中以及其对肝癌细胞的影响探讨其在HCC中的机制。方法: 肝癌组织和相邻非癌的正常组织取自武汉市中医医院的 75 名肝癌患者, 采用定量实时聚合酶链反应(qRT- PCR)法比较miR-193a和 Wilms'肿瘤 1基因(WT1)在肝癌组织和相邻非癌的正常组织的表达水平。探讨肝癌组织中miR-193a的表达与肿瘤分期、肿瘤是否转移、分化程度的相关性。检测肝癌细胞系MHCC97H,HCCLM3,Huh7和人正常肝细胞系LO2中miR-193a表达。此外,过表达和抑制miR-193a后通过细胞计数CCK-8法对HCC细胞增殖进行检测。通过异种移植物肿瘤形成试验观察抑制miR-193a是否加速HCC的肿瘤生长。通过双重荧光素酶测定法证实WT1是否为miR-193a的靶点基因。结果: 相对于非癌组织,肝癌组织中miR-193a相对于表达显著降低(P<0.01),WT1基因表达显著升高,同时miR-193a的表达在正常肝细胞系中的表达显著低于肝癌细胞系(P<0.05~P<0.01)。肝癌患者TNM分期(P=0.004)以及淋巴结转移程度(P=0.013)在miR-193a低表达组患者相对于高于miR-193a高表达组更为严重。生存分析表明低表达miR-193a的肝癌患者的预后较差。此外,发现过表达miR-193a抑制HCC细胞增殖,而抑制miR-193a表达促进了HCC增殖。在体外miR-193a过表达抑制了HCC的肿瘤生长。荧光素酶试验证实WT1是miR-193a的靶点基因。结论: miR-193a可通过靶向WT1抑制HCC发生。
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刘亚琪
郑承红
关键词 肝细胞癌miR-193aWT1    
AbstractObjective: To elucidate the function of miR-193a in HCC. Methods: The expression levels of miR-193a and Wilms' tumor-1 (WT1) were detected by quantitative real Time-polymerase chain Reaction (qRT-PCR) assay. In addition, miR-193a expression was also investigated in MHCC97H,HCCLM3,Huh7 and LO2 cell lines. Moreover, the HCC proliferation was examined through cell counting Kit-8 (CCK-8) assay after miR-193a overexpression or inhibition. Tumor growth of HCC was observed via xenograft tumor formation assay. The relationship between miR-193a and WT1 was verified by the dual-Luciferase assay. Results: Downregulation of miR-193a whereas upregulation of WT1 were identified in HCC, which predicted a worse prognosis in HCC patients. Meanwhile, miR-193a was significantly decreased in LO2 cell lines compared with MHCC97H,HCCLM3,Huh7 cell lines. Low miR-193a expression HCC patients showed severer TMN stage and presence of lymph node metastasis. Furthermore, it was found that miR-193a mimic suppressed cell proliferation by inhibiting WT1. miR-193a also inhibited tumor growth of HCC. Conclusion: miR-193a suppressed tumorigenesis of HCC by targeting WT1.
Key wordsHepatocellular carcinoma    MicroRNA-193a    WT1
    
基金资助:湖北省自然基金项目,[编号:2017(20)]
引用本文:   
刘亚琪, 郑承红. MicroRNA-193a 通过靶向 WT1抑制肝细胞癌发生的机制研究[J]. 河北医学, 2021, 27(1): 1-4.
LIU Yaqi, et al. Mechanism of MicroRNA-193a Suppressing the Tumorigenesis of Hepatocellular Carcinoma Through Targeting WT1. HeBei Med, 2021, 27(1): 1-4.
链接本文:  
http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2021.01.001     或     http://www.hbyxzzs.cn/CN/Y2021/V27/I1/1
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