An Analysis of the Role of Fecal SFRP2 Gene Methylation Combined with Quantitative Immunochemical Fecal Occult Blood Test in Colorectal Cancer Screening
KANG Qian, XIE Hui, WANG Xin, et al
The Seventh Medical Center of PLA General Hospital, Beijing 100700, China
Abstract：Objective: To investigate the clinical significance of SFRP2 gene methylation combined with quantitative immunochemical fecal occult blood test for colorectal cancer screening. Methods: 106 patients with colorectal cancer,106 patients with advanced adenoma and 106 healthy people who underwent colonoscopy and pathology in the hospital's gastroenterology clinic were selected. Fecal samples were collected for Methylation-specific PCR to detect the methylation of fecal SFRP2 gene promoter and quantitative immunochemical fecal occult blood. Positive rate was calculated. Results: The positive rate of fecal SFRP2 gene methylation and positive QFIT were 77.35%,25.47%, 6.60%, 83.96%,15.09%, 1.87% in the colorectal cancer group, advanced adenoma group and control group, respectively. The cancer group and the advanced adenoma group were higher than the control group (P<0.05), and the colorectal cancer group was higher than the advanced adenoma group (P<0.05). The positive rate of SFRP2 methylation combined with QFIT was 91.51%, 52.83% and 5.66% in the colorectal cancer group, advanced adenoma group and control group, respectively. The detection rate of colorectal cancer group and the advanced adenoma group were higher than the individual test. (P<0.05). SFRP2 gene methylation in feces, the sensitivity of fecal occult blood quantification and combined detection were 77.35%, 83.96% and 93.40%,respectively, and the specificities were 93.40%, 98.11% and 93.40%, respectively. The sensitivity of the combined detection were higher than individual test (P<0.05). Conclusion: SFRP2 methylation combined with fecal occult blood quantification could improve the accuracy of screening colorectal cancer and have potential clinical value.
康倩, 谢惠, 王昕, 张杰, 李娜, 盛剑秋. 粪便SFRP2基因甲基化联合定量免疫便潜血检测在结直肠癌筛查的应用[J]. 河北医学, 2020, 26(7): 1097-1099.
KANG Qian, XIE Hui, WANG Xin, et al. An Analysis of the Role of Fecal SFRP2 Gene Methylation Combined with Quantitative Immunochemical Fecal Occult Blood Test in Colorectal Cancer Screening. HeBei Med, 2020, 26(7): 1097-1099.
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2020, Vol. 26 
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