Abstract：Objective: To study the biological role of LncRNA AK093407 in human colorectal cancer cells. Methods: QRT PCR was used to detect the expression of lncRNA AK093407 in human colorectal cancer and HCT-116 cells; siRNA silenced the expression of lncRNA AK093407 in colorectal cancer cells: MTT assay was used to detect cell proliferation; annexin V / PI staining was used to detect cell apoptosis; PI staining was used to detect cell cycle; Western staining was used to detect cell cycle. The expression of Caspase-3, caspase-9, caspase-10, Bax, Bcl-2, p21, p27 and p-STAT3 were detected by blot. Results: The expression of lncRNA AK093407 in human colorectal cancer was higher than that in adjacent tissues; the expression of lncRNA AK093407 in HCT-116 was higher than that in NM460; lncRNA was silenced After AK093407, the proliferation activity of HCT-116 cells decreased, apoptosis increased, cell cycle blocked in G1 / S phase; the expression of caspase 3, caspase 9, caspase 10, Bax, Bcl-2, p21, p27 and p-STAT3 decreased. Conclusion: LncRNA AK093407 is highly expressed in colorectal cancer and HCT-116 cells. Silencing lncRNA AK093407 can inhibit cell proliferation, induce cell apoptosis and cell cycle arrest.
孙奥, 赵学荣, 王建平, 赵恩宏, 常晓敏, 肖丽君. LncRNA AK093407在结直肠癌中的生物学作用研究[J]. 河北医学, 2020, 26(3): 353-357.
SUN Ao, ZHAO Xuerong, WANG Jianping, et al. Research on Biological Role of LncRNA AK093407 in Colorectal Cancer. HeBei Med, 2020, 26(3): 353-357.
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2020, Vol. 26 
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