Abstract：Objective: To explore the relationship between cytomegalovirus infection and serum TNF-alpha, IL-1 and IL-6 in patients with spontaneous abortion. Methods: The clinical data of 110 cases of spontaneous abortion pregnant women (observation group) and 70 cases of normal pregnant women (control group) were collected from January 2017 to January 2019. The observation group was divided into two subgroups according to the detection results of HCMV-IgM, HCMV-DNA and HCMV-RNA in peripheral blood, and either of them was positive. For HCMV active infection, 26 cases were active infection and 84 cases were inactive infection. The positive rates of HCMV-IgM, HCMV-DNA and HCMV-RNA, the levels of inflammatory cytokines (TNF-a, IL-1, IL-6) and the levels of inflammatory cytokines in active infection group and inactive infection group were compared between the observation group and the control group. The relationship between HCMV active infection and TNF-a, IL-1, IL-6 was analyzed. Results: The positive rates of HCMV-IgM, HCMV-DNA and HCMV-RNA in the observation group were 37.37%, 16.36% and 2364% respectively, which were significantly higher than those in the control group (7.14%, 4.29% and 5.71%), the differences were statistically significant (P<0.05). The serum levels of TNF-alpha, IL-1 and IL-6 in active infection group were higher than those in inactive infection group, the differences were statistically significant (P<0.05), it is suggested that serum TNF - α, IL-1 and IL-6 levels are related to active infection, and the higher the level, the higher the risk of active infection. Conclusion: HCMV infection is one of the important causes of spontaneous abortion, and HCMV infection is related to the levels of TNF - α, IL-1 and IL-6. The higher the level, the higher the risk of active infection, may play an important role in the occurrence of abortion.
[1] Donahue J G, Kieke B A, King J P, et al. Association of spontaneous abortion with receipt of inactivated influenza vaccine containing H1N1pdm09 in 2010-11 and 2011-12.[J]. Vaccine, 2017, 35(40):5314. [2] 朱烨. 原因不明自然流产相关因素分析[J].中国计划生育和妇产科,2017,9(10):21~24. [3] Fornara L, Fornara C, Cananzi R, et al. Incidence of human cytomegalovirus (HCMV) infection and T-cell response in psoriatic patients before and during anti-TNF-α therapy.[J]. British Journal of Dermatology, 2017, 177(6):5314~5322. [4] 柯旭,赵卫东,李静,等.人巨细胞病毒活动性感染与自然流产的相关机制研究[J].安徽医科大学学报,2019,54(2):143~147. [5] 陈琼娜,沈凯,黄燕燕,等.Hcmv-miR-UL112荧光定量RT-PCR检测方法的建立[J].医学研究杂志,2016,45(4):53~56. [6] 孙梦,温宗梅.肺移植术后巨细胞病毒感染的研究进展[J].实用临床医学,2018,19(11):108~110,114. [7] 王晗,李廷栋,郭小怡.巨细胞病毒实验室检测方法研究进展及其用于新生儿筛查的可行性[J].临床儿科杂志,2018,36(3):221~226. [8] Simonazzi G, Cervi F, Zavatta A, et al. Congenital cytomegalovirus infection: prognostic value of maternal DNAemia at amniocentesis[J]. Clinical Infectious Diseases An Official Publication of the Infectious Diseases Society of America, 2017, 64(2):207. [9] 张雨,曾慧慧.人巨细胞病毒感染及母婴传播阻断临床研究进展[J].中华实验和临床感染病杂志(电子版),2017,11(6):526~532. [10] 张杰,刘亮,周辉,郭桂喜,于强,银鹏飞,陈文强,苏兴.上调抗增殖蛋白表达对TNF-α诱导的H9c2心肌细胞损伤的影响[J].郑州大学学报(医学版),2019,54(5):728~732. [11] 王丛文,宋钰,丁磊,等.孕妇及其新生儿HSV、EBV和HCMV感染状况的PCR分析[J].现代妇产科进展,2018,27(4):281~283. [12] 孙海严,丁秋允,史义军,等.白细胞介素6在银屑病发病中作用的研究进展[J].医学研究与教育,2016,33(5):44~48. [13] Forte E, Swaminathan S, Schroeder M W, et al. Tumor necrosis factor alpha induces reactivation of human cytomegalovirus independently of myeloid cell differentiation following posttranscriptional establishment of latency[J]. MBio,2018,9(5): e01560.
2020, Vol. 26 
冀公网安备13080202000677号