Abstract：Objective: To research Shenshao Oral Liquid Inhibits Secondary Inflammatory Injure Induced by CD14/TLR4 after Intracerebral Hemorrhage in Experimental Rats. Methods: Experimental rats were randomly divided into sham operation group, model group and intervention group. Each group further was subdivided into 6 h group, 12 h group, 24 h group, 72 h group, and 7 d group. ICH group and SS group were used to duplicate the model of cerebral hemorrhage. SS group was given Shenshao oral liquid (8ml/d) by gavage after waking up. The number and protein expression of CD14, TLR4 and IL-8 positive cells were detected by immunohistochemistry and Western blotting. Results: The edema was appeared after 6h after the ICH model was established, and the water content of brain was gradually increased at 12h to 72h, and reached to the highest level at 72h, then the brain edema was gradually decreased, and ultimately got right original level at 7d. Compared with the ICH group, the water content of brain in the rats of the intervention group was obviously decreased (P<0.05). The expression of CD14 in brain tissue cells of sham group was slight; CD14 protein began to express around hematoma in ICH group at 6h after operation, and a small number of positive cells increased with time, reached the peak at 72h, and then decreased gradually. The trend of TLR4 and IL-8 protein expression was consistent with that of CD14. The expression level of TLR4 and CD14 was positively correlated with brain water content (P<0.05), and the expression level of TLR4 and IL-8 was significantly decreased after Shenshao oral liquid. Conclusion: Shenshao oral liquid may reduce the inducing factors of CD14 / TLR4 Signal pathway, reduce the secondary inflammatory injury of brain tissue around hematoma after cerebral hemorrhage, and play a protective role in brain tissue.
刘宏伟, 唐兴国, 李斌斌, 孟令丽, 秦博文. 参芍口服液对实验性脑出血大鼠脑内CD14/TLR4介导的继发性炎症损伤的干预效果研究[J]. 河北医学, 2020, 26(2): 333-337.
LIU Hongwei, TANG Xingguo, LI Binbin, et al. Effect of Shenshao Oral Liquid on CD14 / TLR4 Mediated Secondary Inflammatory Injury in the Brain of Rats with Experimental Cerebral Hemorrhage. HeBei Med, 2020, 26(2): 333-337.
2020, Vol. 26 
冀公网安备13080202000677号