Abstract：Objective: To study whether sulforaphane (SF) can reduce the acute lung injury (ALI) by up-regulating the transcription factor NF-E2 related factor 2 (Nrf2) to play an antioxidant and anti-inflammatory role. Methods: Thirty-two healthy mice were randomly divided into acute lung injury group (ALI group), control group (control group), SF group, and SF solvent group, 8 mice in each group. The control group was injected intraperitoneally with 10 mg/kg saline. SF group was intraperitoneally injected with 20 mg/kg (SF), SF solvent group was intraperitoneally injected with 20 mg/kg (SF carrier) solvent, 2 twice a day, continuous injection was performed for 3 days, and ALI group, SF group, and SF solvent group were injected with lipopolysaccharide at the 4th day. (LPS) 10 mg/kg, and an endotoxin-induced ALI model was prepared. Six hours after injection of LPS or normal saline, vena cava blood was removed and serum IL-6 and TNF-ɑ levels were measured by ELISA. Pulmonary tissue pathological results were scored for lung injury after observation with light microscope. Nrf2 nucleus in mouse lung tissue Protein expression was measured by Western blotting, and lung dry weight/wet weight ratio was calculated simultaneously. Colorimetric method was used to detect SOD, MPO and iNOS content in lung tissue homogenate. Results: The lung injury score, iNOS, MPO, IL-6, and TNF-ɑ levels in the ALI group and the SF solvent group were significantly higher than those in the control group, and the expression of Nrf2 protein was up-regulated (P<0.05). SOD was lower than that in the control group (P<0.05). There was no significant difference in the above indicators between the ALI group and the SF solvent group (P>0.05). The IL-6, TNF-ɑ, lung injury score, and iNOS content in the SF group were significant. Compared with the ALI group, the lung dry weight/wet weight ratio and SOD content increased, and the expression of Nrf2 protein was up-regulated (P<0.05). Conclusion: SF can reduce the severity of LPS-induced acute lung injury by inducing the expression of Nrf2 protein through SF, and it can also reduce the levels of IL-6, TNF-ɑ and MPO, confirming that Nrf2 transcription factor can effectively protect ALI.
[1] Zimmermann K K, Spassov S G, Strosing K M, et al. Hydrogen sulfide exerts anti-oxidative and anti-inflammatory effects in acute lung injury[J]. Inflammation, 2017, 41(1):249~259. [2] 张敏,许成辰,徐婷贞,等.基于Nrf2/Keap1/p62信号通路探讨百合固金汤对LPS诱导的小鼠急性肺损伤的保护作用[J].中国实验方剂学杂志,2019,25(15):77~82. [3] Shu-Hua Y, Li-Hui Y, Lin L, et al. Protective mechanism of sulforaphane on cadmium-induced sertoli cell injury in mice testis via Nrf2/ARE signaling pathway[J]. Molecules, 2018, 23(7):1774. [4] Badamjav R, Sonom D, Wu Y, et al. The protective effects of thalictrum minus L. on lipopolysaccharide-induced acute lung injury[J]. Journal of Ethnopharmacology, 2019, 248:112355. [5] 谢仰民,陈韩秋,陈穗,等.大鼠急性呼吸窘迫综合征动物模型的建立[J].中国实验动物学报,2002,10(4):243~245. [6] Chen LJ, Ding YB, Ma PL, et al. The protective effect of lidocaine on lipopolysaccharide-induced acute lung injury in rats through NF-κB and p38 MAPK signaling pathway and excessive inflammatory responses[J]. Eur Rev Med Pharmacol Sci,2018,22(7):2099~2108. [7] Meng L, Li L, Lu S, et al. The protective effect of dexmedetomidine on LPS-induced acute lung injury through the HMGB1-mediated TLR4/NF-κB and PI3K/Akt/mTOR pathways[J]. Mol Immunol,2018,94:7~17. [8] Dong-Liang C, Yun-Xin L, Jia-Xing Z, et al. Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression[J]. Theranostics, 2017, 7(19):4836~4849. [9] Liu J X, Li X, Yan F G, et al. Protective effect of forsythoside B against lipopolysaccharide-induced acute lung injury by attenuating the TLR4/NF-κB pathway[J]. International Immunopharmacology, 2019, 66:336~346. [10] Hu X, Li H, Fu L, et al. The protective effect of hyperin on LPS-induced acute lung injury in mice[J]. Microbial Pathogenesis, 2019, 127:116~120. [11] Bian X, Liu X, Liu J, et al. Hepatoprotective effect of chiisanoside from acanthopanax sessiliflorus against LPS/D-GalN-induced acute liver injury by inhibiting NF-κB and activating Nrf2/HO-1 signaling pathways[J]. Journal of the Science of Food and Agriculture, 2019, 99(7):3283~3290.
2020, Vol. 26 
冀公网安备13080202000677号