Abstract：Objective: To study the expression levels of microRNA-34a (miR-34a), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in serum of children with craniocerebral injury and their significance. Methods: 78 children with craniocerebral injury admitted to Qinghai Women and Children's Hospital from September 2017 to August 2019 were divided into mild group (23 cases), moderate group (30 cases) and severe group (25 cases) according to Glasgow Coma score(GCS); at the same time, 80 healthy controls in the same period were selected. Real-time quantitative PCR (qRT-PCR) was used to detect the level of miR-34a in serum; the level of NO in serum was detected by chemical colorimetry; and enzyme-linked immunosorbent assay (ELISA) was used to detect the level of TNF-α in serum. Pearson method was used to analyze the correlation between serum miR-34a, NO, TNF-α and GCS score. Receiver operating characteristic curve (ROC) was applied to analyze the predictive value of serum miR-34a, NO, TNF-α levels for TBI. Results: ①The levels of serum miR-34a, NO and TNF-α in children with craniocerebral injury were higher than those in the control group, and the differences were significant (P<0.05); ②The levels of serum miR-34a, NO and TNF-α in severe group were higher than those in moderate group and mild group, the levels of serum miR-34a, NO and TNF-α in moderate group were higher than those in mild group, and the differences were significant (P<0.05); ③Pearson analysis showed that levels of serum miR-34a, NO and TNF-α were negatively correlated with GCS scores in children with craniocerebral injury (P<0.05); ④The area under the curve of TBI predicted by serum miR-34a, NO and TNF-α levels is 0.821(95%CI:0.675~0.967), 0.902(95%CI:0.816~0.989), 0.851(95%CI:0.741~0.961); cutoff values are 0.65, 38.45 μmol/L, 153.49 pg/mL; sensitivity is 88.9%,94.4%,72.2%; specificity is 72.0%, 70.0%, 98.0%; The area under the curve predicted by the joint detection is 0.998 (95%CI: 0.992~1.000); the sensitivity is 100.0%; and the specificity is 98.0%; ⑤The levels of serum miR-34a, NO and TNF-α in the death group were significantly higher than those in the survival group, and the differences were significant (P<0.05). Conclusions: miR-34a, NO, and TNF-α are all highly expressed in the serum of children with craniocerebral injury, which is closely related to the degree of craniocerebral injury. Joint detection has certain predictive value, and it has a certain degree of assessment on the grim prognosis of children with craniocerebral injury.
汪生毅, 孙小红, 王新法. 颅脑损伤患儿血清中miR-34a和NO TNF-α表达及意义[J]. 河北医学, 2020, 26(12): 1947-1951.
WANG Shengyi, SUN Xiaohong, et al. Expressions and Significance of Serum MiR-34a NO and TNF-α in Children with Craniocerebral Injury. HeBei Med, 2020, 26(12): 1947-1951.
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2020, Vol. 26 
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