特应性皮炎患者血清Linc00324和Th17细胞因子的表达及意义

doi:10.3969/j.issn.1006-6233.2020.11.06

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摘要 目的: 检测特应性皮炎患者血清长链非编码RNA00324(Linc00324)和辅助性T细胞17(Th17)因子的表达情况,探讨其在特应性皮炎发病中的作用及关系。方法: 选取2017年10月至2019年11月在本院经病理确诊的80例特应性皮炎患者为研究对象,分为特应性皮炎轻度组26例,特应性皮炎中度组24例,特应性皮炎重度组30例,同时选取35例健康人群作为对照组。采用实时荧光定量PCR法(qRT-PCR)检测各组血清Linc00324表达水平;采用酶联免疫吸附试验(ELISA)法对Th17细胞因子白细胞介素-4(IL-4)、白细胞介素-10(IL-10)水平进行检测;采用Pearson法分析特应性皮炎患者血清Linc00324及Th17细胞因子IL-4、IL-10表达水平的相关性。结果: 特应性皮炎患者血清Linc00324水平、Th17细胞因子IL-4、IL-10水平均显著高于对照组(P<0.05),重度组、中度组特应性皮炎患者血清Linc00324水平、Th17细胞因子IL-4、IL-10水平均显著高于轻度组(P<0.05),重度组特应性皮炎患者血清Linc00324水平、Th17细胞因子IL-4、IL-10水平均显著高于中度组(P<0.05)。特应性皮炎患者血清Linc00324表达水平与Th17细胞因子IL-4、IL-10水平呈明显正相关(r=0.516、r=0.498,P<0.05)。结论: Linc00324在特应性皮炎患者血清中明显高表达,Th17细胞因子IL-4、IL-10水平在特应性皮炎患者中明显高表达,且与特应性皮炎的发生和病情的发展密切相关,提示Linc00324可作为特应性皮炎早期诊断及病情评估的潜在生物学指标,具有重要的临床意义。

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	运蓓蕾
	林伟
	刘悦

关键词 ：特应性皮炎, 长链非编码RNA00324, 白细胞介素-4, 白细胞介素-10

Abstract：Objective: To detect the expression of serum long non-coding RNA 00324 (Linc00324) and T helper cell 17(Th17) cytokine in patients with atopic dermatitis and to explore its role and relationship in the pathogenesis of atopic dermatitis.Methods: 80 patients with atopic dermatitis confirmed by pathology in our hospital from October 2017 to November 2019 were selected as the study objects,which were divided into 26 patients with mild atopic dermatitis,24 patients with moderate atopic dermatitis,30 patients with severe atopic dermatitis,and 35 healthy people were selected as the control group.The expression of Linc00324 in serum was detected by real-time fluorescent quantitative PCR (qRT PCR).The levels of interleukin-4(IL-4) and interleukin-10(IL-10) in Th17 cells were detected by enzyme-linked immunosorbent assay (ELISA),and Pearson method was used to analyze the correlations between serum Linc00324 and the expression levels of Th17 cytokines IL-4,IL-10 in patients with atopic dermatitis.Results: The levels of serum Linc00324,Th17 cytokines IL-4 and IL-10 in patients with atopic dermatitis were significantly higher than those in the control group (P<0.05),the levels of serum Linc00324,Th17 cytokines IL-4 and IL-10 in severe group and moderate group were significantly higher than those in mild group (P<0.05),and the levels of serum Linc00324,Th17 cytokines IL-4 and IL-10 in severe group were significantly higher than those in moderate group (P<0.05).The expression of Linc00324 in serum of patients with atopic dermatitis was positively correlated with the levels of Th17 cytokines IL-4 and IL-10 (r = 0.516,r = 0.498,P<0.05).Conclusion: Linc00324 is highly expressed in the serum of patients with atopic dermatitis,the levels of Th17 cytokines IL-4 and IL-10 are significantly higher in patients with atopic dermatitis,which are closely related to the occurrence and development of atopic dermatitis.It is suggested that Linc00324 can be used as a potential biological index for the early diagnosis and assessment of atopic dermatitis with important clinical significance.

Key words： Atopic dermatitis Long-chain non-coding

基金资助:辽宁省科学技术计划项目,(编号:2018223179)

引用本文:

运蓓蕾, 林伟, 刘悦. 特应性皮炎患者血清Linc00324和Th17细胞因子的表达及意义[J]. 河北医学, 2020, 26(11): 1785-1789.
YUN Beilei, LIN Wei, LIU Yue. Expression and Significance of Linc00324 and Th17 Cytokines in Serum of Patients with Atopic Dermatitis. HeBei Med, 2020, 26(11): 1785-1789.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2020.11.06 或 http://www.hbyxzzs.cn/CN/Y2020/V26/I11/1785

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